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Recombinant Human SS18 293 Cell Lysate

Cat.No.: SS18-1468HCL
Description: Antigen standard for synovial sarcoma translocation, chromosome 18 (SS18), transcript variant 1 is a lysate prepared from HEK293T cells transiently transfected with a TrueORF gene-carrying pCMV plasmid and then lysed in RIPA Buffer. Protein concentration was determined using a colorimetric assay. The antigen control carries a C-terminal Myc/DDK tag for detection.
Source: HEK 293 cells
Species: Human
Components: This product includes 3 vials: 1 vial of gene-specific cell lysate, 1 vial of control vector cell lysate, and 1 vial of loading buffer. Each lysate vial contains 0.1 mg lysate in 0.1 ml (1 mg/ml) of RIPA Buffer (50 mM Tris-HCl pH7.5, 250 mM NaCl, 5 mM EDTA, 50 mM NaF, 1% NP40). The loading buffer vial contains 0.5 ml 2X SDS Loading Buffer (125 mM Tris-Cl, pH6.8, 10% glycerol, 4% SDS, 0.002% Bromophenol blue, 5% beta-mercaptoethanol).
Size: 0.1 mg
Storage Instruction: Store at -80°C. Minimize freeze-thaw cycles. After addition of 2X SDS Loading Buffer, the lysates can be stored at -20°C. Product is guaranteed 6 months from the date of shipment.
Applications: ELISA, WB, IP. WB: Mix equal volume of lysates with 2X SDS Loading Buffer. Boil the mixture for 10 min before loading (for membrane protein lysates, incubate the mixture at room temperature for 30 min). Load 5 ug lysate per lane.
Gene Name: SS18 synovial sarcoma translocation, chromosome 18 [ Homo sapiens ]
Official Symbol: SS18
Synonyms: SS18; synovial sarcoma translocation, chromosome 18; SSXT; protein SSXT; SYT; synovial sarcoma, translocated to X chromosome; synovial sarcoma translocated to X chromosome protein; MGC116875;
Gene ID: 6760
mRNA Refseq: NM_001007559
Protein Refseq: NP_001007560
MIM: 600192
UniProt ID: Q15532
Chromosome Location: 18q11.2
Pathway: Transcriptional misregulation in cancer, organism-specific biosystem; Transcriptional misregulation in cancer, conserved biosystem;

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