"SRY" Related Products

Recombinant Human SRY 293 Cell Lysate

Cat.No.: SRY-1469HCL
Description: Antigen standard for sex determining region Y (SRY) is a lysate prepared from HEK293T cells transiently transfected with a TrueORF gene-carrying pCMV plasmid and then lysed in RIPA Buffer. Protein concentration was determined using a colorimetric assay. The antigen control carries a C-terminal Myc/DDK tag for detection.
Source: HEK 293 cells
Species: Human
Components: This product includes 3 vials: 1 vial of gene-specific cell lysate, 1 vial of control vector cell lysate, and 1 vial of loading buffer. Each lysate vial contains 0.1 mg lysate in 0.1 ml (1 mg/ml) of RIPA Buffer (50 mM Tris-HCl pH7.5, 250 mM NaCl, 5 mM EDTA, 50 mM NaF, 1% NP40). The loading buffer vial contains 0.5 ml 2X SDS Loading Buffer (125 mM Tris-Cl, pH6.8, 10% glycerol, 4% SDS, 0.002% Bromophenol blue, 5% beta-mercaptoethanol).
Size: 0.1 mg
Storage Instruction: Store at -80°C. Minimize freeze-thaw cycles. After addition of 2X SDS Loading Buffer, the lysates can be stored at -20°C. Product is guaranteed 6 months from the date of shipment.
Applications: ELISA, WB, IP. WB: Mix equal volume of lysates with 2X SDS Loading Buffer. Boil the mixture for 10 min before loading (for membrane protein lysates, incubate the mixture at room temperature for 30 min). Load 5 ug lysate per lane.
Gene Name: SRY sex determining region Y [ Homo sapiens ]
Official Symbol: SRY
Synonyms: SRY; sex determining region Y; sex-determining region Y protein; TDF; testis determining factor; testis-determining factor; sex-determining region on Y; sex determining region protein; essential protein for sex determination in human males; TDY; SRXX1; SRXY1;
Gene ID: 6736
mRNA Refseq: NM_003140
Protein Refseq: NP_003131
MIM: 480000
UniProt ID: Q05066
Chromosome Location: Yp11.3
Pathway: Androgen Receptor Signaling Pathway, organism-specific biosystem; Regulation of Androgen receptor activity, organism-specific biosystem;

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