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Recombinant Human SRPK1 293 Cell Lysate

Cat.No. : SRPK1-1475HCL
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  • Gene Information
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Description : Antigen standard for SFRS protein kinase 1 (SRPK1) is a lysate prepared from HEK293T cells transiently transfected with a TrueORF gene-carrying pCMV plasmid and then lysed in RIPA Buffer. Protein concentration was determined using a colorimetric assay. The antigen control carries a C-terminal Myc/DDK tag for detection.
Source : HEK 293 cells
Species : Human
Components : This product includes 3 vials: 1 vial of gene-specific cell lysate, 1 vial of control vector cell lysate, and 1 vial of loading buffer. Each lysate vial contains 0.1 mg lysate in 0.1 ml (1 mg/ml) of RIPA Buffer (50 mM Tris-HCl pH7.5, 250 mM NaCl, 5 mM EDTA, 50 mM NaF, 1% NP40). The loading buffer vial contains 0.5 ml 2X SDS Loading Buffer (125 mM Tris-Cl, pH6.8, 10% glycerol, 4% SDS, 0.002% Bromophenol blue, 5% beta-mercaptoethanol).
Size : 0.1 mg
Storage Instruction : Store at -80°C. Minimize freeze-thaw cycles. After addition of 2X SDS Loading Buffer, the lysates can be stored at -20°C. Product is guaranteed 6 months from the date of shipment.
Applications : ELISA, WB, IP. WB: Mix equal volume of lysates with 2X SDS Loading Buffer. Boil the mixture for 10 min before loading (for membrane protein lysates, incubate the mixture at room temperature for 30 min). Load 5 ug lysate per lane.
Gene Name : SRPK1 SRSF protein kinase 1 [ Homo sapiens ]
Official Symbol : SRPK1
Synonyms : SRPK1; SRSF protein kinase 1; SFRS protein kinase 1; serine/arginine rich splicing factor kinase 1; SFRSK1; SR protein kinase 1; SR-protein-specific kinase 1; serine/threonine-protein kinase SRPK1; serine/arginine-rich splicing factor kinase 1;
Gene ID : 6732
mRNA Refseq : NM_003137
Protein Refseq : NP_003128
MIM : 601939
UniProt ID : Q96SB4
Chromosome Location : 6p21.31
Pathway : Herpes simplex infection, organism-specific biosystem; Herpes simplex infection, conserved biosystem; mRNA processing, organism-specific biosystem;

For Research Use Only. Not intended for any clinical use. No products from Creative BioMart may be resold, modified for resale or used to manufacture commercial products without prior written approval from Creative BioMart.

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Q&As (5)

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Can SRPK1 inhibition be used in combination with other cancer treatments? 02/22/2022

Combination therapy with SRPK1 inhibitors and other cancer treatments, such as chemotherapy or immunotherapy, is being explored for enhanced efficacy.

How does SRPK1 influence RNA splicing? 04/03/2021

SRPK1 phosphorylates SR proteins, which affects their splicing activity and can lead to the inclusion or exclusion of specific exons in mRNA transcripts.

What other diseases or conditions have been linked to SRPK1? 01/10/2018

SRPK1 dysregulation has been implicated in neurodegenerative diseases, cardiovascular disorders, and autoimmune conditions.

Are there any known genetic mutations related to SRPK1 and disease susceptibility? 05/25/2017

Some genetic variations in the SRPK1 gene have been associated with increased susceptibility to certain diseases, but further research is needed to establish these connections.

How does SRPK1 play a role in RNA processing disorders? 01/11/2017

SRPK1 abnormalities can lead to errors in RNA splicing, contributing to RNA processing disorders, such as spinal muscular atrophy and myotonic dystrophy.

Customer Reviews (3)

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Reviews
02/02/2023

    By using SRPK1 protein, I can gain insights into the underlying molecular processes contributing to amyloidosis, which can ultimately aid in the development of novel therapeutic strategies.

    10/17/2022

      The manufacturer plays a crucial role in supporting my research with their extensive expertise and resources.

      01/01/2022

        This assurance enables me to confidently utilize the SRPK1 protein in my research, knowing that it will consistently deliver reliable and accurate results.

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