"SPTLC2" Related Products

Recombinant Human SPTLC2 293 Cell Lysate

Cat.No.: SPTLC2-1484HCL
Description: Antigen standard for serine palmitoyltransferase, long chain base subunit 2 (SPTLC2) is a lysate prepared from HEK293T cells transiently transfected with a TrueORF gene-carrying pCMV plasmid and then lysed in RIPA Buffer. Protein concentration was determined using a colorimetric assay. The antigen control carries a C-terminal Myc/DDK tag for detection.
Source: HEK 293 cells
Species: Human
Components: This product includes 3 vials: 1 vial of gene-specific cell lysate, 1 vial of control vector cell lysate, and 1 vial of loading buffer. Each lysate vial contains 0.1 mg lysate in 0.1 ml (1 mg/ml) of RIPA Buffer (50 mM Tris-HCl pH7.5, 250 mM NaCl, 5 mM EDTA, 50 mM NaF, 1% NP40). The loading buffer vial contains 0.5 ml 2X SDS Loading Buffer (125 mM Tris-Cl, pH6.8, 10% glycerol, 4% SDS, 0.002% Bromophenol blue, 5% beta-mercaptoethanol).
Size: 0.1 mg
Storage Instruction: Store at -80°C. Minimize freeze-thaw cycles. After addition of 2X SDS Loading Buffer, the lysates can be stored at -20°C. Product is guaranteed 6 months from the date of shipment.
Applications: ELISA, WB, IP. WB: Mix equal volume of lysates with 2X SDS Loading Buffer. Boil the mixture for 10 min before loading (for membrane protein lysates, incubate the mixture at room temperature for 30 min). Load 5 ug lysate per lane.
Gene Name: SPTLC2 serine palmitoyltransferase, long chain base subunit 2 [ Homo sapiens ]
Official Symbol: SPTLC2
Synonyms: SPTLC2; serine palmitoyltransferase, long chain base subunit 2; serine palmitoyltransferase 2; hLCB2a; KIAA0526; LCB2; LCB2A; LCB 2; SPT 2; serine-palmitoyl-CoA transferase 2; long chain base biosynthesis protein 2a; serine palmitoyltransferase, subunit II; SPT2; HSN1C; NSAN1C;
Gene ID: 9517
mRNA Refseq: NM_004863
Protein Refseq: NP_004854
MIM: 605713
UniProt ID: O15270
Chromosome Location: 14q24.3
Pathway: Ceramide biosynthesis, organism-specific biosystem; Ceramide biosynthesis, conserved biosystem; Metabolic pathways, organism-specific biosystem; Metabolism, organism-specific biosystem; Metabolism of lipids and lipoproteins, organism-specific biosystem; Sphingolipid de novo biosynthesis, organism-specific biosystem; Sphingolipid metabolism, organism-specific biosystem;

Online Inquiry

Note: There will be extra charge for optional service!

Please input "biomart" as verification code. Please review Creative BioMart's privacy policy for more information

Optional requirements on this protein    +Expand

Price Inquiry

Welcome! For price inquiries, please feel free to contact us through the form below. We will get back to you as soon as possible.