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Recombinant Human SOCS2 293 Cell Lysate

Cat.No.: SOCS2-1581HCL
Description: Antigen standard for suppressor of cytokine signaling 2 (SOCS2) is a lysate prepared from HEK293T cells transiently transfected with a TrueORF gene-carrying pCMV plasmid and then lysed in RIPA Buffer. Protein concentration was determined using a colorimetric assay. The antigen control carries a C-terminal Myc/DDK tag for detection.
Source: HEK 293 cells
Species: Human
Components: This product includes 3 vials: 1 vial of gene-specific cell lysate, 1 vial of control vector cell lysate, and 1 vial of loading buffer. Each lysate vial contains 0.1 mg lysate in 0.1 ml (1 mg/ml) of RIPA Buffer (50 mM Tris-HCl pH7.5, 250 mM NaCl, 5 mM EDTA, 50 mM NaF, 1% NP40). The loading buffer vial contains 0.5 ml 2X SDS Loading Buffer (125 mM Tris-Cl, pH6.8, 10% glycerol, 4% SDS, 0.002% Bromophenol blue, 5% beta-mercaptoethanol).
Size: 0.1 mg
Storage Instruction: Store at -80°C. Minimize freeze-thaw cycles. After addition of 2X SDS Loading Buffer, the lysates can be stored at -20°C. Product is guaranteed 6 months from the date of shipment.
Applications: ELISA, WB, IP. WB: Mix equal volume of lysates with 2X SDS Loading Buffer. Boil the mixture for 10 min before loading (for membrane protein lysates, incubate the mixture at room temperature for 30 min). Load 5 ug lysate per lane.
Gene Name: SOCS2 suppressor of cytokine signaling 2 [ Homo sapiens ]
Official Symbol: SOCS2
Synonyms: SOCS2; suppressor of cytokine signaling 2; CIS2; Cish2; SOCS 2; SSI 2; SSI2; STAT induced STAT inhibitor 2; STATI2; CIS-2; STAT induced STAT inhibitor-2; STAT-induced STAT inhibitor 2; STAT-induced STAT inhibitor-2; cytokine-inducible SH2 protein 2; suppressor of cytokine signaling-2; SSI-2; SOCS-2;
Gene ID: 8835
mRNA Refseq: NM_003877
Protein Refseq: NP_003868
MIM: 605117
UniProt ID: O14508
Chromosome Location: 12q
Pathway: Cytokine Signaling in Immune system, organism-specific biosystem; ECS complex, organism-specific biosystem; ECS complex, conserved biosystem; Growth hormone receptor signaling, organism-specific biosystem; IL-3 Signaling Pathway, organism-specific biosystem; IL2-mediated signaling events, organism-specific biosystem; Immune System, organism-specific biosystem;
Function: JAK pathway signal transduction adaptor activity; SH3/SH2 adaptor activity; growth hormone receptor binding; insulin-like growth factor receptor binding; prolactin receptor binding; protein binding;

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