"SNRNP25" Related Products

Recombinant Human SNRNP25 293 Cell Lysate

Cat.No.: SNRNP25-1625HCL
Description: Antigen standard for small nuclear ribonucleoprotein 25kDa (U11/U12) (SNRNP25) is a lysate prepared from HEK293T cells transiently transfected with a TrueORF gene-carrying pCMV plasmid and then lysed in RIPA Buffer. Protein concentration was determined using a colorimetric assay. The antigen control carries a C-terminal Myc/DDK tag for detection.
Source: HEK 293 cells
Species: Human
Components: This product includes 3 vials: 1 vial of gene-specific cell lysate, 1 vial of control vector cell lysate, and 1 vial of loading buffer. Each lysate vial contains 0.1 mg lysate in 0.1 ml (1 mg/ml) of RIPA Buffer (50 mM Tris-HCl pH7.5, 250 mM NaCl, 5 mM EDTA, 50 mM NaF, 1% NP40). The loading buffer vial contains 0.5 ml 2X SDS Loading Buffer (125 mM Tris-Cl, pH6.8, 10% glycerol, 4% SDS, 0.002% Bromophenol blue, 5% beta-mercaptoethanol).
Size: 0.1 mg
Storage Instruction: Store at -80°C. Minimize freeze-thaw cycles. After addition of 2X SDS Loading Buffer, the lysates can be stored at -20°C. Product is guaranteed 6 months from the date of shipment.
Applications: ELISA, WB, IP. WB: Mix equal volume of lysates with 2X SDS Loading Buffer. Boil the mixture for 10 min before loading (for membrane protein lysates, incubate the mixture at room temperature for 30 min). Load 5 ug lysate per lane.
Gene Name: SNRNP25 small nuclear ribonucleoprotein 25kDa (U11/U12) [ Homo sapiens ]
Official Symbol: SNRNP25
Synonyms: SNRNP25; small nuclear ribonucleoprotein 25kDa (U11/U12); C16orf33, chromosome 16 open reading frame 33; U11/U12 small nuclear ribonucleoprotein 25 kDa protein; U11/U12 snRNP 25K; minus-99 protein; U11/U12 snRNP 25 kDa protein; C16orf33; FLJ22940;
Gene ID: 79622
mRNA Refseq: NM_024571
Protein Refseq: NP_078847
UniProt ID: Q9BV90
Chromosome Location: 16p13.3

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