Recombinant Human SLK 293 Cell Lysate

• Cat.No.: SLK-1681HCL

Specification

• Description: Antigen standard for STE20-like kinase (yeast) (SLK) is a lysate prepared from HEK293T cells transiently transfected with a TrueORF gene-carrying pCMV plasmid and then lysed in RIPA Buffer. Protein concentration was determined using a colorimetric assay. The antigen control carries a C-terminal Myc/DDK tag for detection.
• Source: HEK 293 cells
• Species: Human
• Components: This product includes 3 vials: 1 vial of gene-specific cell lysate, 1 vial of control vector cell lysate, and 1 vial of loading buffer. Each lysate vial contains 0.1 mg lysate in 0.1 ml (1 mg/ml) of RIPA Buffer (50 mM Tris-HCl pH7.5, 250 mM NaCl, 5 mM EDTA, 50 mM NaF, 1% NP40). The loading buffer vial contains 0.5 ml 2X SDS Loading Buffer (125 mM Tris-Cl, pH6.8, 10% glycerol, 4% SDS, 0.002% Bromophenol blue, 5% beta-mercaptoethanol).
• Size: 0.1 mg
• Storage Instruction: Store at -80°C. Minimize freeze-thaw cycles. After addition of 2X SDS Loading Buffer, the lysates can be stored at -20°C. Product is guaranteed 6 months from the date of shipment.
• Applications: ELISA, WB, IP. WB: Mix equal volume of lysates with 2X SDS Loading Buffer. Boil the mixture for 10 min before loading (for membrane protein lysates, incubate the mixture at room temperature for 30 min). Load 5 ug lysate per lane.

Gene Information

• Gene Name: SLK STE20-like kinase [ Homo sapiens ]
• Official Symbol: SLK
• Synonyms: SLK; STE20-like kinase; SNF1 (sucrose nonfermenting, yeast, homolog) like kinase, SNF1 sucrose nonfermenting like kinase (yeast) , STE20 like kinase (yeast); STE20-like serine/threonine-protein kinase; KIAA0204; se20 9; STK2; hSLK; STE20-related kinase; Long Ste20-like Kinase; CTCL tumor antigen se20-9; serine/threonine-protein kinase 2; Ste20-related serine/threonine kinase; SNF1 sucrose nonfermenting like kinase; STE20-related serine/threonine-protein kinase; SNF1 (sucrose nonfermenting, yeast, homolog)-like kinase, SNF1 sucrose nonfermenting like kinase; LOSK; se20-9; bA16H23.1; MGC133067
• Gene ID: 9748
• mRNA Refseq: NM_014720
• Protein Refseq: NP_055535
• UniProt ID: Q9H2G2
• Chromosome Location: 10q25.1
• Pathway: Oocyte meiosis, organism-specific biosystem; Oocyte meiosis, conserved biosystem
• Function: ATP binding; DNA binding; nuclease activity; nucleotide binding; protein homodimerization activity; protein serine/threonine kinase activity