Recombinant Human SLCO1A2 293 Cell Lysate

• Cat.No.: SLCO1A2-1691HCL

Specification

• Description: Antigen standard for solute carrier organic anion transporter family, member 1A2 (SLCO1A2), transcript variant 2 is a lysate prepared from HEK293T cells transiently transfected with a TrueORF gene-carrying pCMV plasmid and then lysed in RIPA Buffer. Protein concentration was determined using a colorimetric assay. The antigen control carries a C-terminal Myc/DDK tag for detection.
• Source: HEK 293 cells
• Species: Human
• Components: This product includes 3 vials: 1 vial of gene-specific cell lysate, 1 vial of control vector cell lysate, and 1 vial of loading buffer. Each lysate vial contains 0.1 mg lysate in 0.1 ml (1 mg/ml) of RIPA Buffer (50 mM Tris-HCl pH7.5, 250 mM NaCl, 5 mM EDTA, 50 mM NaF, 1% NP40). The loading buffer vial contains 0.5 ml 2X SDS Loading Buffer (125 mM Tris-Cl, pH6.8, 10% glycerol, 4% SDS, 0.002% Bromophenol blue, 5% beta-mercaptoethanol).
• Size: 0.1 mg
• Storage Instruction: Store at -80°C. Minimize freeze-thaw cycles. After addition of 2X SDS Loading Buffer, the lysates can be stored at -20°C. Product is guaranteed 6 months from the date of shipment.
• Applications: ELISA, WB, IP. WB: Mix equal volume of lysates with 2X SDS Loading Buffer. Boil the mixture for 10 min before loading (for membrane protein lysates, incubate the mixture at room temperature for 30 min). Load 5 ug lysate per lane.

Gene Information

• Gene Name: SLCO1A2 solute carrier organic anion transporter family, member 1A2 [ Homo sapiens ]
• Official Symbol: SLCO1A2
• Synonyms: SLCO1A2; solute carrier organic anion transporter family, member 1A2; SLC21A3, solute carrier family 21 (organic anion transporter), member 3; solute carrier organic anion transporter family member 1A2; OATP; OATP A; OATP1A2; OATP-1; solute carrier family 21 member 3; organic anion transporting polypeptide A; organic anion-transporting polypeptide 1; sodium-independent organic anion transporter; solute carrier family 21 (organic anion transporter), member 3; OATP-A; SLC21A3
• Gene ID: 6579
• mRNA Refseq: NM_021094
• Protein Refseq: NP_066580
• MIM: 602883
• UniProt ID: P46721
• Chromosome Location: 12p12
• Pathway: Bile acid and bile salt metabolism, organism-specific biosystem; Bile secretion, organism-specific biosystem; Bile secretion, conserved biosystem; Metabolism, organism-specific biosystem; Metabolism of lipids and lipoproteins, organism-specific biosystem; Recycling of bile acids and salts, organism-specific biosystem; SLC-mediated transmembrane transport, organism-specific biosystem
• Function: organic anion transmembrane transporter activity; transporter activity