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Recombinant Human SLC5A1 293 Cell Lysate

Cat.No.: SLC5A1-1710HCL
Description: Antigen standard for solute carrier family 5 (sodium/glucose cotransporter), member 1 (SLC5A1) is a lysate prepared from HEK293T cells transiently transfected with a TrueORF gene-carrying pCMV plasmid and then lysed in RIPA Buffer. Protein concentration was determined using a colorimetric assay. The antigen control carries a C-terminal Myc/DDK tag for detection.
Source: HEK 293 cells
Species: Human
Components: This product includes 3 vials: 1 vial of gene-specific cell lysate, 1 vial of control vector cell lysate, and 1 vial of loading buffer. Each lysate vial contains 0.1 mg lysate in 0.1 ml (1 mg/ml) of RIPA Buffer (50 mM Tris-HCl pH7.5, 250 mM NaCl, 5 mM EDTA, 50 mM NaF, 1% NP40). The loading buffer vial contains 0.5 ml 2X SDS Loading Buffer (125 mM Tris-Cl, pH6.8, 10% glycerol, 4% SDS, 0.002% Bromophenol blue, 5% beta-mercaptoethanol).
Size: 0.1 mg
Storage Instruction: Store at -80°C. Minimize freeze-thaw cycles. After addition of 2X SDS Loading Buffer, the lysates can be stored at -20°C. Product is guaranteed 6 months from the date of shipment.
Applications: ELISA, WB, IP. WB: Mix equal volume of lysates with 2X SDS Loading Buffer. Boil the mixture for 10 min before loading (for membrane protein lysates, incubate the mixture at room temperature for 30 min). Load 5 ug lysate per lane.
Gene Name: SLC5A1 solute carrier family 5 (sodium/glucose cotransporter), member 1 [ Homo sapiens ]
Official Symbol: SLC5A1
Synonyms: SLC5A1; solute carrier family 5 (sodium/glucose cotransporter), member 1; SGLT1; sodium/glucose cotransporter 1; D22S675; NAGT; Na+/glucose cotransporter 1; high affinity sodium-glucose cotransporter;
Gene ID: 6523
mRNA Refseq: NM_000343
Protein Refseq: NP_000334
MIM: 182380
UniProt ID: P13866
Chromosome Location: 22q12.3
Pathway: Bile secretion, organism-specific biosystem; Bile secretion, conserved biosystem; Carbohydrate digestion and absorption, organism-specific biosystem; Carbohydrate digestion and absorption, conserved biosystem; Hexose transport, organism-specific biosystem; Metabolism, organism-specific biosystem; Metabolism of carbohydrates, organism-specific biosystem;
Function: glucose:sodium symporter activity; protein binding; symporter activity; transporter activity;

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