Recombinant Human SLC26A3 293 Cell Lysate

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Recombinant Human SLC26A3 293 Cell Lysate

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Cat.No. : SLC26A3-1753HCL
Description : Antigen standard for solute carrier family 26, member 3 (SLC26A3) is a lysate prepared from HEK293T cells transiently transfected with a TrueORF gene-carrying pCMV plasmid and then lysed in RIPA Buffer. Protein concentration was determined using a colorimetric assay. The antigen control carries a C-terminal Myc/DDK tag for detection.
Source : HEK 293 cells
Species : Human
Components : This product includes 3 vials: 1 vial of gene-specific cell lysate, 1 vial of control vector cell lysate, and 1 vial of loading buffer. Each lysate vial contains 0.1 mg lysate in 0.1 ml (1 mg/ml) of RIPA Buffer (50 mM Tris-HCl pH7.5, 250 mM NaCl, 5 mM EDTA, 50 mM NaF, 1% NP40). The loading buffer vial contains 0.5 ml 2X SDS Loading Buffer (125 mM Tris-Cl, pH6.8, 10% glycerol, 4% SDS, 0.002% Bromophenol blue, 5% beta-mercaptoethanol).
Size : 0.1 mg
Storage Instruction : Store at -80°C. Minimize freeze-thaw cycles. After addition of 2X SDS Loading Buffer, the lysates can be stored at -20°C. Product is guaranteed 6 months from the date of shipment.
Applications : ELISA, WB, IP. WB: Mix equal volume of lysates with 2X SDS Loading Buffer. Boil the mixture for 10 min before loading (for membrane protein lysates, incubate the mixture at room temperature for 30 min). Load 5 ug lysate per lane.
Gene Name : SLC26A3 solute carrier family 26, member 3 [ Homo sapiens ]
Official Symbol : SLC26A3
Synonyms : SLC26A3; solute carrier family 26, member 3; CLD, congenital chloride diarrhea , DRA; chloride anion exchanger; down-regulated in adenoma protein; CLD; DRA;
Gene ID : 1811
mRNA Refseq : NM_000111
Protein Refseq : NP_000102
MIM : 126650
UniProt ID : P40879
Chromosome Location : 7q31
Pathway : Mineral absorption, organism-specific biosystem; Mineral absorption, conserved biosystem; Multifunctional anion exchangers, organism-specific biosystem; Pancreatic secretion, organism-specific biosystem; Pancreatic secretion, conserved biosystem; SLC-mediated transmembrane transport, organism-specific biosystem; Transmembrane transport of small molecules, organism-specific biosystem;
Function : anion:anion antiporter activity; antiporter activity; secondary active sulfate transmembrane transporter activity; sequence-specific DNA binding transcription factor activity; transcription cofactor activity; transporter activity;

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