Recombinant Human SLC12A5 293 Cell Lysate

• Cat.No.: SLC12A5-1805HCL

Specification

• Description: Antigen standard for solute carrier family 12 (potassium-chloride transporter), member 5 (SLC12A5), transcript variant 2 is a lysate prepared from HEK293T cells transiently transfected with a TrueORF gene-carrying pCMV plasmid and then lysed in RIPA Buffer. Protein concentration was determined using a colorimetric assay. The antigen control carries a C-terminal Myc/DDK tag for detection.
• Source: HEK 293 cells
• Species: Human
• Components: This product includes 3 vials: 1 vial of gene-specific cell lysate, 1 vial of control vector cell lysate, and 1 vial of loading buffer. Each lysate vial contains 0.1 mg lysate in 0.1 ml (1 mg/ml) of RIPA Buffer (50 mM Tris-HCl pH7.5, 250 mM NaCl, 5 mM EDTA, 50 mM NaF, 1% NP40). The loading buffer vial contains 0.5 ml 2X SDS Loading Buffer (125 mM Tris-Cl, pH6.8, 10% glycerol, 4% SDS, 0.002% Bromophenol blue, 5% beta-mercaptoethanol).
• Size: 0.1 mg
• Storage Instruction: Store at -80°C. Minimize freeze-thaw cycles. After addition of 2X SDS Loading Buffer, the lysates can be stored at -20°C. Product is guaranteed 6 months from the date of shipment.
• Applications: ELISA, WB, IP. WB: Mix equal volume of lysates with 2X SDS Loading Buffer. Boil the mixture for 10 min before loading (for membrane protein lysates, incubate the mixture at room temperature for 30 min). Load 5 ug lysate per lane.

Gene Information

• Gene Name: SLC12A5 solute carrier family 12 (potassium/chloride transporter), member 5 [ Homo sapiens ]
• Official Symbol: SLC12A5
• Synonyms: SLC12A5; solute carrier family 12 (potassium/chloride transporter), member 5; solute carrier family 12 member 5; KCC2; KIAA1176; hKCC2; K-Cl cotransporter 2; neuronal K-Cl cotransporter; erythroid K-Cl cotransporter 2; electroneutral potassium-chloride cotransporter 2
• Gene ID: 57468
• mRNA Refseq: NM_001134771
• Protein Refseq: NP_001128243
• MIM: 606726
• UniProt ID: Q9H2X9
• Chromosome Location: 20q13.12
• Pathway: Cation-coupled Chloride cotransporters, organism-specific biosystem; GABAergic synapse, organism-specific biosystem; GABAergic synapse, conserved biosystem; SLC-mediated transmembrane transport, organism-specific biosystem; Transmembrane transport of small molecules, organism-specific biosystem; Transport of inorganic cations/anions and amino acids/oligopeptides, organism-specific biosystem
• Function: ammonia transmembrane transporter activity; potassium:chloride symporter activity; symporter activity; transporter activity