"SLC12A4" Related Products

Recombinant Human SLC12A4 293 Cell Lysate

Cat.No.: SLC12A4-1806HCL
Description: Antigen standard for solute carrier family 12 (potassium/chloride transporters), member 4 (SLC12A4), transcript variant 1 is a lysate prepared from HEK293T cells transiently transfected with a TrueORF gene-carrying pCMV plasmid and then lysed in RIPA Buffer. Protein concentration was determined using a colorimetric assay. The antigen control carries a C-terminal Myc/DDK tag for detection.
Source: HEK 293 cells
Species: Human
Components: This product includes 3 vials: 1 vial of gene-specific cell lysate, 1 vial of control vector cell lysate, and 1 vial of loading buffer. Each lysate vial contains 0.1 mg lysate in 0.1 ml (1 mg/ml) of RIPA Buffer (50 mM Tris-HCl pH7.5, 250 mM NaCl, 5 mM EDTA, 50 mM NaF, 1% NP40). The loading buffer vial contains 0.5 ml 2X SDS Loading Buffer (125 mM Tris-Cl, pH6.8, 10% glycerol, 4% SDS, 0.002% Bromophenol blue, 5% beta-mercaptoethanol).
Size: 0.1 mg
Storage Instruction: Store at -80°C. Minimize freeze-thaw cycles. After addition of 2X SDS Loading Buffer, the lysates can be stored at -20°C. Product is guaranteed 6 months from the date of shipment.
Applications: ELISA, WB, IP. WB: Mix equal volume of lysates with 2X SDS Loading Buffer. Boil the mixture for 10 min before loading (for membrane protein lysates, incubate the mixture at room temperature for 30 min). Load 5 ug lysate per lane.
Gene Name: SLC12A4 solute carrier family 12 (potassium/chloride transporters), member 4 [ Homo sapiens ]
Official Symbol: SLC12A4
Synonyms: SLC12A4; solute carrier family 12 (potassium/chloride transporters), member 4; solute carrier family 12 member 4; KCC1; hKCC1; K-Cl cotransporter; erythroid K:Cl cotransporter; erythroid K-Cl cotransporter 1; potassium/chloride cotransporter 1; electroneutral potassium-chloride cotransporter 1; FLJ17069; FLJ40489;
Gene ID: 6560
mRNA Refseq: NM_001145961
Protein Refseq: NP_001139433
MIM: 604119
UniProt ID: Q9UP95
Chromosome Location: 16q22.1
Pathway: Cation-coupled Chloride cotransporters, organism-specific biosystem; SLC-mediated transmembrane transport, organism-specific biosystem; Transmembrane transport of small molecules, organism-specific biosystem; Transport of inorganic cations/anions and amino acids/oligopeptides, organism-specific biosystem;
Function: potassium:chloride symporter activity; symporter activity; transporter activity;

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