"SH3BGRL" Related Products


Recombinant Human SH3BGRL 293 Cell Lysate

Cat.No.: SH3BGRL-1874HCL
Description: Antigen standard for SH3 domain binding glutamic acid-rich protein like (SH3BGRL) is a lysate prepared from HEK293T cells transiently transfected with a TrueORF gene-carrying pCMV plasmid and then lysed in RIPA Buffer. Protein concentration was determined using a colorimetric assay. The antigen control carries a C-terminal Myc/DDK tag for detection.
Source: HEK 293 cells
Species: Human
Components: This product includes 3 vials: 1 vial of gene-specific cell lysate, 1 vial of control vector cell lysate, and 1 vial of loading buffer. Each lysate vial contains 0.1 mg lysate in 0.1 ml (1 mg/ml) of RIPA Buffer (50 mM Tris-HCl pH7.5, 250 mM NaCl, 5 mM EDTA, 50 mM NaF, 1% NP40). The loading buffer vial contains 0.5 ml 2X SDS Loading Buffer (125 mM Tris-Cl, pH6.8, 10% glycerol, 4% SDS, 0.002% Bromophenol blue, 5% beta-mercaptoethanol).
Size: 0.1 mg
Storage Instruction: Store at -80°C. Minimize freeze-thaw cycles. After addition of 2X SDS Loading Buffer, the lysates can be stored at -20°C. Product is guaranteed 6 months from the date of shipment.
Applications: ELISA, WB, IP. WB: Mix equal volume of lysates with 2X SDS Loading Buffer. Boil the mixture for 10 min before loading (for membrane protein lysates, incubate the mixture at room temperature for 30 min). Load 5 ug lysate per lane.
Gene Name: SH3BGRL SH3 domain binding glutamic acid-rich protein like [ Homo sapiens ]
Official Symbol: SH3BGRL
Synonyms: SH3BGRL; SH3 domain binding glutamic acid-rich protein like; SH3 domain-binding glutamic acid-rich-like protein; MGC117402; SH3-binding domain glutamic acid-rich protein like; SH3BGR;
Gene ID: 6451
mRNA Refseq: NM_003022
Protein Refseq: NP_003013
MIM: 300190
UniProt ID: O75368
Chromosome Location: Xq13.3
Pathway: EGFR1 Signaling Pathway, organism-specific biosystem;
Function: SH3 domain binding; SH3/SH2 adaptor activity;

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