"SH2B3" Related Products

Recombinant Human SH2B3 293 Cell Lysate

Cat.No.: SH2B3-1880HCL
Description: Antigen standard for SH2B adaptor protein 3 (SH2B3) is a lysate prepared from HEK293T cells transiently transfected with a TrueORF gene-carrying pCMV plasmid and then lysed in RIPA Buffer. Protein concentration was determined using a colorimetric assay. The antigen control carries a C-terminal Myc/DDK tag for detection.
Source: HEK 293 cells
Species: Human
Components: This product includes 3 vials: 1 vial of gene-specific cell lysate, 1 vial of control vector cell lysate, and 1 vial of loading buffer. Each lysate vial contains 0.1 mg lysate in 0.1 ml (1 mg/ml) of RIPA Buffer (50 mM Tris-HCl pH7.5, 250 mM NaCl, 5 mM EDTA, 50 mM NaF, 1% NP40). The loading buffer vial contains 0.5 ml 2X SDS Loading Buffer (125 mM Tris-Cl, pH6.8, 10% glycerol, 4% SDS, 0.002% Bromophenol blue, 5% beta-mercaptoethanol).
Size: 0.1 mg
Storage Instruction: Store at -80°C. Minimize freeze-thaw cycles. After addition of 2X SDS Loading Buffer, the lysates can be stored at -20°C. Product is guaranteed 6 months from the date of shipment.
Applications: ELISA, WB, IP. WB: Mix equal volume of lysates with 2X SDS Loading Buffer. Boil the mixture for 10 min before loading (for membrane protein lysates, incubate the mixture at room temperature for 30 min). Load 5 ug lysate per lane.
Gene Name: SH2B3 SH2B adaptor protein 3 [ Homo sapiens ]
Official Symbol: SH2B3
Synonyms: SH2B3; SH2B adaptor protein 3; SH2B adapter protein 3; IDDM20; LNK; lymphocyte adaptor protein; signal transduction protein Lnk; lymphocyte-specific adapter protein Lnk;
Gene ID: 10019
mRNA Refseq: NM_005475
Protein Refseq: NP_005466
MIM: 605093
UniProt ID: Q9UQQ2
Chromosome Location: 12q24.12
Pathway: EPO signaling pathway, organism-specific biosystem; Factors involved in megakaryocyte development and platelet production, organism-specific biosystem; Hemostasis, organism-specific biosystem; Neurotrophin signaling pathway, organism-specific biosystem; Neurotrophin signaling pathway, conserved biosystem; Regulation of KIT signaling, organism-specific biosystem; Signal Transduction, organism-specific biosystem;
Function: signal transducer activity;

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