Recombinant Human SFRS13A 293 Cell Lysate

• Cat.No.: SRSF10-1906HCL

Specification

• Description: Antigen standard for splicing factor, arginine/serine-rich 13A (SFRS13A), transcript variant 1 is a lysate prepared from HEK293T cells transiently transfected with a TrueORF gene-carrying pCMV plasmid and then lysed in RIPA Buffer. Protein concentration was determined using a colorimetric assay. The antigen control carries a C-terminal Myc/DDK tag for detection.
• Source: HEK 293 cells
• Species: Human
• Components: This product includes 3 vials: 1 vial of gene-specific cell lysate, 1 vial of control vector cell lysate, and 1 vial of loading buffer. Each lysate vial contains 0.1 mg lysate in 0.1 ml (1 mg/ml) of RIPA Buffer (50 mM Tris-HCl pH7.5, 250 mM NaCl, 5 mM EDTA, 50 mM NaF, 1% NP40). The loading buffer vial contains 0.5 ml 2X SDS Loading Buffer (125 mM Tris-Cl, pH6.8, 10% glycerol, 4% SDS, 0.002% Bromophenol blue, 5% beta-mercaptoethanol).
• Size: 0.1 mg
• Storage Instruction: Store at -80°C. Minimize freeze-thaw cycles. After addition of 2X SDS Loading Buffer, the lysates can be stored at -20°C. Product is guaranteed 6 months from the date of shipment.
• Applications: ELISA, WB, IP. WB: Mix equal volume of lysates with 2X SDS Loading Buffer. Boil the mixture for 10 min before loading (for membrane protein lysates, incubate the mixture at room temperature for 30 min). Load 5 ug lysate per lane.

Gene Information

• Gene Name: SRSF10 serine/arginine-rich splicing factor 10 [ Homo sapiens ]
• Official Symbol: SRSF10
• Synonyms: NSSR; TASR; SRp38; TASR1; TASR2; FUSIP1; FUSIP2; SFRS13; SRrp40; SFRS13A; PPP1R149; serine/arginine-rich splicing factor 10; SR splicing factor 10; splicing factor SRp38; TLS-associated SR protein; neural-salient SR protein; 40 kDa SR-repressor protein; TLS-associated protein TASR; TLS-associated protein with SR repeats; TLS-associated serine-arginine protein 1; TLS-associated serine-arginine protein 2; splicing factor, arginine/serine-rich 13; splicing factor, arginine/serine-rich 13A; serine-arginine repressor protein (40 kDa); TLS-associated protein with Ser-Arg repeats; protein phosphatase 1, regulatory subunit 149; FUS interacting protein (serine-arginine rich) 1; FUS-interacting protein (serine-arginine rich) 2
• Gene ID: 10772
• mRNA Refseq: NM_006625
• Protein Refseq: NP_006616
• MIM: 605221
• UniProt ID: O75494
• Chromosome Location: 1p36.11
• Pathway: Spliceosome, organism-specific biosystem; Spliceosome, conserved biosystem; mRNA processing, organism-specific biosystem
• Function: RNA binding; RS domain binding; nucleotide binding