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Recombinant Human SEC61G 293 Cell Lysate

Cat.No. : SEC61G-1989HCL
  • Specification
  • Gene Information
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Description : Antigen standard for Sec61 gamma subunit (SEC61G), transcript variant 1 is a lysate prepared from HEK293T cells transiently transfected with a TrueORF gene-carrying pCMV plasmid and then lysed in RIPA Buffer. Protein concentration was determined using a colorimetric assay. The antigen control carries a C-terminal Myc/DDK tag for detection.
Source : HEK 293 cells
Species : Human
Components : This product includes 3 vials: 1 vial of gene-specific cell lysate, 1 vial of control vector cell lysate, and 1 vial of loading buffer. Each lysate vial contains 0.1 mg lysate in 0.1 ml (1 mg/ml) of RIPA Buffer (50 mM Tris-HCl pH7.5, 250 mM NaCl, 5 mM EDTA, 50 mM NaF, 1% NP40). The loading buffer vial contains 0.5 ml 2X SDS Loading Buffer (125 mM Tris-Cl, pH6.8, 10% glycerol, 4% SDS, 0.002% Bromophenol blue, 5% beta-mercaptoethanol).
Size : 0.1 mg
Storage Instruction : Store at -80°C. Minimize freeze-thaw cycles. After addition of 2X SDS Loading Buffer, the lysates can be stored at -20°C. Product is guaranteed 6 months from the date of shipment.
Applications : ELISA, WB, IP. WB: Mix equal volume of lysates with 2X SDS Loading Buffer. Boil the mixture for 10 min before loading (for membrane protein lysates, incubate the mixture at room temperature for 30 min). Load 5 ug lysate per lane.
Gene Name : SEC61G Sec61 gamma subunit [ Homo sapiens ]
Official Symbol : SEC61G
Synonyms : SEC61G; Sec61 gamma subunit; protein transport protein Sec61 subunit gamma; SSS1; protein transport protein SEC61 gamma subunit;
Gene ID : 23480
mRNA Refseq : NM_001012456
Protein Refseq : NP_001012474
MIM : 609215
UniProt ID : P60059
Chromosome Location : 7p11.2
Pathway : Adaptive Immune System, organism-specific biosystem; Antigen processing-Cross presentation, organism-specific biosystem; Class I MHC mediated antigen processing and presentation, organism-specific biosystem; ER-Phagosome pathway, organism-specific biosystem; Gene Expression, organism-specific biosystem; Immune System, organism-specific biosystem;
Function : P-P-bond-hydrolysis-driven protein transmembrane transporter activity; protein transporter activity;

For Research Use Only. Not intended for any clinical use. No products from Creative BioMart may be resold, modified for resale or used to manufacture commercial products without prior written approval from Creative BioMart.

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