"RIPK2" Related Products

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Recombinant Human RIPK2 293 Cell Lysate

Cat.No.: RIPK2-2333HCL
Description: Antigen standard for receptor-interacting serine-threonine kinase 2 (RIPK2) is a lysate prepared from HEK293T cells transiently transfected with a TrueORF gene-carrying pCMV plasmid and then lysed in RIPA Buffer. Protein concentration was determined using a colorimetric assay. The antigen control carries a C-terminal Myc/DDK tag for detection.
Source: HEK 293 cells
Species: Human
Components: This product includes 3 vials: 1 vial of gene-specific cell lysate, 1 vial of control vector cell lysate, and 1 vial of loading buffer. Each lysate vial contains 0.1 mg lysate in 0.1 ml (1 mg/ml) of RIPA Buffer (50 mM Tris-HCl pH7.5, 250 mM NaCl, 5 mM EDTA, 50 mM NaF, 1% NP40). The loading buffer vial contains 0.5 ml 2X SDS Loading Buffer (125 mM Tris-Cl, pH6.8, 10% glycerol, 4% SDS, 0.002% Bromophenol blue, 5% beta-mercaptoethanol).
Size: 0.1 mg
Storage Instruction: Store at -80°C. Minimize freeze-thaw cycles. After addition of 2X SDS Loading Buffer, the lysates can be stored at -20°C. Product is guaranteed 6 months from the date of shipment.
Applications: ELISA, WB, IP. WB: Mix equal volume of lysates with 2X SDS Loading Buffer. Boil the mixture for 10 min before loading (for membrane protein lysates, incubate the mixture at room temperature for 30 min). Load 5 ug lysate per lane.
Gene Name: RIPK2 receptor-interacting serine-threonine kinase 2 [ Homo sapiens ]
Official Symbol: RIPK2
Synonyms: RIPK2; receptor-interacting serine-threonine kinase 2; receptor-interacting serine/threonine-protein kinase 2; CARD3; CARDIAK; RICK; RIP2; RIP-2; CARD-carrying kinase; growth-inhibiting gene 30; tyrosine-protein kinase RIPK2; receptor interacting protein 2; receptor-interacting protein 2; RIP-like-interacting CLARP kinase; CARD-containing IL-1 beta ICE-kinase; CARD-containing interleukin-1 beta-converting enzyme-associated kinase; CARD-containing interleukin-1 beta-converting enzyme (ICE)-associated kinase; receptor-interacting protein (RIP)-like interacting caspase-like apoptosis regulatory protein (CLARP) kinase; CCK; GIG30;
Gene ID: 8767
mRNA Refseq: NM_003821
Protein Refseq: NP_003812
MIM: 603455
UniProt ID: O43353
Chromosome Location: 8q21
Pathway: Activated TLR4 signalling, organism-specific biosystem; Adaptive Immune System, organism-specific biosystem; Canonical NF-kappaB pathway, organism-specific biosystem; Cytokine Signaling in Immune system, organism-specific biosystem; Downstream TCR signaling, organism-specific biosystem; FAS pathway and Stress induction of HSP regulation, organism-specific biosystem; IL12-mediated signaling events, organism-specific biosystem;
Function: ATP binding; CARD domain binding; CARD domain binding; LIM domain binding; non-membrane spanning protein tyrosine kinase activity; nucleotide binding; protein binding; protein homodimerization activity; protein serine/threonine kinase activity; signal transducer activity;

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