Recombinant Human RCVRN 293 Cell Lysate

• Cat.No.: RCVRN-2442HCL

Specification

• Description: Antigen standard for recoverin (RCVRN) is a lysate prepared from HEK293T cells transiently transfected with a TrueORF gene-carrying pCMV plasmid and then lysed in RIPA Buffer. Protein concentration was determined using a colorimetric assay. The antigen control carries a C-terminal Myc/DDK tag for detection.
• Source: HEK 293 cells
• Species: Human
• Components: This product includes 3 vials: 1 vial of gene-specific cell lysate, 1 vial of control vector cell lysate, and 1 vial of loading buffer. Each lysate vial contains 0.1 mg lysate in 0.1 ml (1 mg/ml) of RIPA Buffer (50 mM Tris-HCl pH7.5, 250 mM NaCl, 5 mM EDTA, 50 mM NaF, 1% NP40). The loading buffer vial contains 0.5 ml 2X SDS Loading Buffer (125 mM Tris-Cl, pH6.8, 10% glycerol, 4% SDS, 0.002% Bromophenol blue, 5% beta-mercaptoethanol).
• Size: 0.1 mg
• Storage Instruction: Store at -80°C. Minimize freeze-thaw cycles. After addition of 2X SDS Loading Buffer, the lysates can be stored at -20°C. Product is guaranteed 6 months from the date of shipment.
• Applications: ELISA, WB, IP. WB: Mix equal volume of lysates with 2X SDS Loading Buffer. Boil the mixture for 10 min before loading (for membrane protein lysates, incubate the mixture at room temperature for 30 min). Load 5 ug lysate per lane.

Gene Information

• Gene Name: RCVRN recoverin [ Homo sapiens ]
• Official Symbol: RCVRN
• Synonyms: RCVRN; recoverin; RCV1; cancer associated retinopathy antigen; cancer-associated retinopathy protein
• Gene ID: 5957
• mRNA Refseq: NM_002903
• Protein Refseq: NP_002894
• MIM: 179618
• UniProt ID: P35243
• Chromosome Location: 17p13.1
• Pathway: Phototransduction, organism-specific biosystem; Phototransduction, conserved biosystem
• Function: calcium ion binding; calcium sensitive guanylate cyclase activator activity

Reviews

02/16/2020

Whether investigating metabolic pathways, studying enzymatic activity, or exploring drug targets, this protein offers versatility and reliability, allowing for accurate and meaningful data collection.

05/29/2018

In addition to the remarkable protein quality, the manufacturer provides excellent technical support, proving to be a valuable resource when troubleshooting experimental challenges.

06/24/2016

Incorporating this protein into scientific investigations undoubtedly enhances the reliability and effectiveness of research endeavors.

Q&As

Are there any specific populations that may benefit more from RCVRN-targeted therapies?

Individuals with genetic mutations affecting RCVRN and those with early-stage retinal diseases may potentially benefit more from targeted therapies.

How does the expression of RCVRN change with age, and does this have implications for age-related vision disorders?

The expression of RCVRN may change with age, and understanding these changes can provide insights into age-related vision disorders.

How is the level of RCVRN expression correlated with the progression of certain retinal disorders?

Changes in RCVRN expression levels may correlate with the progression of retinal disorders, serving as a potential indicator of disease severity.

Are there ongoing clinical trials exploring the use of RCVRN-based therapies?

Some clinical trials are investigating the safety and efficacy of gene therapies targeting the RCVRN gene for treating specific eye conditions.

How might the manipulation of RCVRN expression offer therapeutic benefits for patients with vision impairments?

Modulating RCVRN expression could potentially restore or enhance photoreceptor function, providing therapeutic benefits for vision-impaired individuals.