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Recombinant Human RALA 293 Cell Lysate

Cat.No. : RALA-2544HCL
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  • Gene Information
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Description : Antigen standard for v-ral simian leukemia viral oncogene homolog A (ras related) (RALA) is a lysate prepared from HEK293T cells transiently transfected with a TrueORF gene-carrying pCMV plasmid and then lysed in RIPA Buffer. Protein concentration was determined using a colorimetric assay. The antigen control carries a C-terminal Myc/DDK tag for detection.
Source : HEK 293 cells
Species : Human
Components : This product includes 3 vials: 1 vial of gene-specific cell lysate, 1 vial of control vector cell lysate, and 1 vial of loading buffer. Each lysate vial contains 0.1 mg lysate in 0.1 ml (1 mg/ml) of RIPA Buffer (50 mM Tris-HCl pH7.5, 250 mM NaCl, 5 mM EDTA, 50 mM NaF, 1% NP40). The loading buffer vial contains 0.5 ml 2X SDS Loading Buffer (125 mM Tris-Cl, pH6.8, 10% glycerol, 4% SDS, 0.002% Bromophenol blue, 5% beta-mercaptoethanol).
Size : 0.1 mg
Storage Instruction : Store at -80°C. Minimize freeze-thaw cycles. After addition of 2X SDS Loading Buffer, the lysates can be stored at -20°C. Product is guaranteed 6 months from the date of shipment.
Applications : ELISA, WB, IP. WB: Mix equal volume of lysates with 2X SDS Loading Buffer. Boil the mixture for 10 min before loading (for membrane protein lysates, incubate the mixture at room temperature for 30 min). Load 5 ug lysate per lane.
Gene Name : RALA v-ral simian leukemia viral oncogene homolog A (ras related) [ Homo sapiens ]
Official Symbol : RALA
Synonyms : RALA; v-ral simian leukemia viral oncogene homolog A (ras related); RAL; ras-related protein Ral-A; Ras family small GTP binding protein RALA; RAS like protein A; Ras related protein Ral A; RAS-like protein A; MGC48949;
Gene ID : 5898
mRNA Refseq : NM_005402
Protein Refseq : NP_005393
MIM : 179550
UniProt ID : P11233
Chromosome Location : 7p22-p15
Pathway : Arf6 trafficking events, organism-specific biosystem; ErbB1 downstream signaling, organism-specific biosystem; NGF signalling via TRKA from the plasma membrane, organism-specific biosystem; Pancreatic cancer, organism-specific biosystem; Pancreatic cancer, conserved biosystem; Pathways in cancer, organism-specific biosystem; Signal Transduction, organism-specific biosystem;
Function : Edg-2 lysophosphatidic acid receptor binding; GTP binding; GTPase activity; nucleotide binding; protein binding;

For Research Use Only. Not intended for any clinical use. No products from Creative BioMart may be resold, modified for resale or used to manufacture commercial products without prior written approval from Creative BioMart.

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Q&As (7)

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What potential therapeutic applications arise from targeting RALA in disease treatment? 04/06/2023

Targeting RALA could offer new strategies for cancer therapy and other diseases involving signaling abnormalities.

How does RALA contribute to vesicle trafficking? 01/09/2022

It facilitates vesicle trafficking, essential for intracellular transport and communication.

How does RALA interact with other signaling proteins? 08/30/2020

RALA interacts with multiple signaling molecules, coordinating diverse cellular processes.

What is the primary role of RALA in cellular signaling? 09/12/2019

RALA, a small GTPase, is crucial for regulating signal transduction pathways in cells.

How do genetic variations in RALA affect cellular communication? 06/09/2019

Genetic mutations in RALA can disrupt signal transduction, affecting cell behavior.

What is the impact of altered RALA activity on cancer progression? 10/04/2018

Altered RALA activity is linked to cancer development and metastasis.

What role does RALA play in gene expression? 03/29/2017

RALA plays a role in controlling gene expression, influencing various cellular functions.

Customer Reviews (3)

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Reviews
04/06/2023

    Top-notch analysis, a critical asset in our research.

    08/30/2020

      Trusted for results accuracy, elevates our research quality.

      10/04/2018

        Saves time and resources in our protein experiments.

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