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Recombinant Human RAB10 293 Cell Lysate

Cat.No. : RAB10-2632HCL
  • Specification
  • Gene Information
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Description : Antigen standard for RAB10, member RAS oncogene family (RAB10) is a lysate prepared from HEK293T cells transiently transfected with a TrueORF gene-carrying pCMV plasmid and then lysed in RIPA Buffer. Protein concentration was determined using a colorimetric assay. The antigen control carries a C-terminal Myc/DDK tag for detection.
Source : HEK 293 cells
Species : Human
Components : This product includes 3 vials: 1 vial of gene-specific cell lysate, 1 vial of control vector cell lysate, and 1 vial of loading buffer. Each lysate vial contains 0.1 mg lysate in 0.1 ml (1 mg/ml) of RIPA Buffer (50 mM Tris-HCl pH7.5, 250 mM NaCl, 5 mM EDTA, 50 mM NaF, 1% NP40). The loading buffer vial contains 0.5 ml 2X SDS Loading Buffer (125 mM Tris-Cl, pH6.8, 10% glycerol, 4% SDS, 0.002% Bromophenol blue, 5% beta-mercaptoethanol).
Size : 0.1 mg
Storage Instruction : Store at -80°C. Minimize freeze-thaw cycles. After addition of 2X SDS Loading Buffer, the lysates can be stored at -20°C. Product is guaranteed 6 months from the date of shipment.
Applications : ELISA, WB, IP. WB: Mix equal volume of lysates with 2X SDS Loading Buffer. Boil the mixture for 10 min before loading (for membrane protein lysates, incubate the mixture at room temperature for 30 min). Load 5 ug lysate per lane.
Gene Name : RAB10 RAB10, member RAS oncogene family [ Homo sapiens ]
Official Symbol : RAB10
Synonyms : RAB10; RAB10, member RAS oncogene family; ras-related protein Rab-10; ras related GTP binding protein; GTP-binding protein RAB10; ras-related GTP-binding protein;
Gene ID : 10890
mRNA Refseq : NM_016131
Protein Refseq : NP_057215
MIM : 612672
UniProt ID : P61026
Chromosome Location : 2p23.3
Function : GTP binding; nucleotide binding;

For Research Use Only. Not intended for any clinical use. No products from Creative BioMart may be resold, modified for resale or used to manufacture commercial products without prior written approval from Creative BioMart.

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Q&As (8)

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Did you check the phosphorylation status of the produced protein? 02/13/2023

We didn't check the phosphorylation status of our RAB10 proteins. But the following one expressed in HEK293 should be phosphorylated.

How does RAB10 contribute to the process of autophagy and cellular homeostasis? 08/12/2022

RAB10 aids in autophagy, maintaining cellular health by regulating the degradation and recycling of cell components.

What role does RAB10 play in insulin-stimulated glucose uptake and metabolic regulation? 01/27/2021

RAB10 plays a crucial role in glucose uptake and metabolism, particularly in response to insulin.

What are the mechanisms by which RAB10 modulates cell signaling and communication? 10/03/2020

RAB10 regulates cell signaling pathways, playing a role in intercellular communication and response to stimuli.

How does RAB10 influence the development and progression of neurodegenerative diseases? 05/12/2020

RAB10 is linked to neurodegenerative diseases, potentially influencing protein aggregation and neuronal function.

How do alterations in RAB10 expression or activity affect the pathogenesis of diabetes and insulin resistance? 05/04/2019

Variations in RAB10 can contribute to the development of diabetes and insulin resistance by affecting glucose metabolism.

How does RAB10 (Ras-Related Protein Rab-10) facilitate intracellular vesicle trafficking and membrane transport? 01/02/2019

RAB10 is key in directing vesicle trafficking, ensuring efficient transport and membrane dynamics within cells.

What is the impact of RAB10 on endocytic pathways and receptor recycling? 08/07/2018

RAB10 impacts endocytosis and receptor trafficking, affecting cell surface receptor dynamics and signaling.

Customer Reviews (3)

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Reviews
04/12/2021

    Reliable mass spectrometry analysis, pivotal for advancing our proteomics research.

    07/07/2019

      Accurate protein structural analysis, indispensable for our investigations.

      10/17/2018

        Swift and reliable protein sequencing, a key asset for our work.

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