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Recombinant Human PTEN 293 Cell Lysate

Cat.No. : PTEN-2721HCL
  • Specification
  • Gene Information
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Description : Antigen standard for phosphatase and tensin homolog (PTEN) is a lysate prepared from HEK293T cells transiently transfected with a TrueORF gene-carrying pCMV plasmid and then lysed in RIPA Buffer. Protein concentration was determined using a colorimetric assay. The antigen control carries a C-terminal Myc/DDK tag for detection.
Source : HEK 293 cells
Species : Human
Components : This product includes 3 vials: 1 vial of gene-specific cell lysate, 1 vial of control vector cell lysate, and 1 vial of loading buffer. Each lysate vial contains 0.1 mg lysate in 0.1 ml (1 mg/ml) of RIPA Buffer (50 mM Tris-HCl pH7.5, 250 mM NaCl, 5 mM EDTA, 50 mM NaF, 1% NP40). The loading buffer vial contains 0.5 ml 2X SDS Loading Buffer (125 mM Tris-Cl, pH6.8, 10% glycerol, 4% SDS, 0.002% Bromophenol blue, 5% beta-mercaptoethanol).
Size : 0.1 mg
Storage Instruction : Store at -80°C. Minimize freeze-thaw cycles. After addition of 2X SDS Loading Buffer, the lysates can be stored at -20°C. Product is guaranteed 6 months from the date of shipment.
Applications : ELISA, WB, IP. WB: Mix equal volume of lysates with 2X SDS Loading Buffer. Boil the mixture for 10 min before loading (for membrane protein lysates, incubate the mixture at room temperature for 30 min). Load 5 ug lysate per lane.
Gene Name : PTEN phosphatase and tensin homolog [ Homo sapiens ]
Official Symbol : PTEN
Synonyms : PTEN; phosphatase and tensin homolog; BZS, MHAM; phosphatidylinositol-3,4,5-trisphosphate 3-phosphatase and dual-specificity protein phosphatase PTEN; MMAC1; mutated in multiple advanced cancers 1; PTEN1; TEP1; phosphatase and tensin-like protein; MMAC1 phosphatase and tensin homolog deleted on chromosome 10; BZS; DEC; GLM2; MHAM; 10q23del; MGC11227;
Gene ID : 5728
mRNA Refseq : NM_000314
Protein Refseq : NP_000305
UniProt ID : P60484
Chromosome Location : 10q23
Pathway : 3-phosphoinositide degradation, organism-specific biosystem; 3-phosphoinositide degradation, conserved biosystem; Adaptive Immune System, organism-specific biosystem; Androgen Receptor Signaling Pathway, organism-specific biosystem; BCR signaling pathway, organism-specific biosystem; Class I PI3K signaling events, organism-specific biosystem; D-myo-inositol (1,3,4)-trisphosphate biosynthesis, organism-specific biosystem;
Function : PDZ domain binding; anaphase-promoting complex binding; enzyme binding; hydrolase activity; inositol-1,3,4,5-tetrakisphosphate 3-phosphatase activity; lipid binding; magnesium ion binding; phosphatidylinositol-3,4,5-trisphosphate 3-phosphatase activity; phosphatidylinositol-3,4,5-trisphosphate 3-phosphatase activity; phosphatidylinositol-3,4-bisphosphate 3-phosphatase activity; phosphatidylinositol-3-phosphatase activity; phosphoprotein phosphatase activity; protein binding; protein serine/threonine phosphatase activity; protein tyrosine phosphatase activity; protein tyrosine/serine/threonine phosphatase activity;

For Research Use Only. Not intended for any clinical use. No products from Creative BioMart may be resold, modified for resale or used to manufacture commercial products without prior written approval from Creative BioMart.

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Q&As (7)

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Apart from its lipid phosphatase activity, what additional function has been attributed to PTEN? 10/01/2022

In addition to its lipid phosphatase activity, PTEN has an additional function known as a scaffolding protein that contributes to its tumor-suppressive role.

In which cellular compartment does PTEN primarily exert its lipid phosphatase function? 01/02/2022

PTEN primarily exerts its lipid phosphatase function in the cytoplasm, where it regulates the PI3K/AKT pathway.

What is the significance of the FBXO22-mediated degradation of nuclear PTEN in promoting tumorigenesis? 03/30/2020

The significance of FBXO22-mediated degradation of nuclear PTEN in promoting tumorigenesis involves the loss of PTEN's tumor-suppressive functions, leading to uncontrolled cell growth.

How is the expression of nuclear PTEN (核PTEN) regulated, especially in the context of tumorigenesis? 08/22/2019

The expression of nuclear PTEN (核PTEN) is regulated, especially in the context of tumorigenesis, through mechanisms involving post-translational modifications and subcellular localization.

What differences are observed in the expression patterns of nuclear PTEN and cytoplasmic PTEN (浆PTEN) in various cancer processes? 07/25/2019

Differences in expression patterns of nuclear PTEN and cytoplasmic PTEN in various cancer processes include alterations in nuclear PTEN levels contributing to tumorigenesis.

What is the classical function of the PTEN protein encoded by the PTEN gene? 04/03/2019

The classical function of the PTEN protein is to regulate cell growth and prevent tumor formation by suppressing the PI3K/AKT signaling pathway.

What role does nuclear PTEN play in mediating the non-phosphatase-dependent function? 10/28/2018

Nuclear PTEN plays a role in mediating the non-phosphatase-dependent function related to genomic stability and DNA repair.

Customer Reviews (3)

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Reviews
11/05/2022

    Gel electrophoresis consistently displayed well-defined bands, affirming the product's purity and reliability.

    01/18/2021

      The insights gained from the product's application justified its cost, marking a prudent investment in our research endeavors.

      12/20/2020

        Comprehensive documentation streamlined the integration of the product into our experimental workflow.

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