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Recombinant Human PSMD2 293 Cell Lysate

Cat.No. : PSMD2-2750HCL
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  • Gene Information
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Description : Antigen standard for proteasome (prosome, macropain) 26S subunit, non-ATPase, 2 (PSMD2) is a lysate prepared from HEK293T cells transiently transfected with a TrueORF gene-carrying pCMV plasmid and then lysed in RIPA Buffer. Protein concentration was determined using a colorimetric assay. The antigen control carries a C-terminal Myc/DDK tag for detection.
Source : HEK 293 cells
Species : Human
Components : This product includes 3 vials: 1 vial of gene-specific cell lysate, 1 vial of control vector cell lysate, and 1 vial of loading buffer. Each lysate vial contains 0.1 mg lysate in 0.1 ml (1 mg/ml) of RIPA Buffer (50 mM Tris-HCl pH7.5, 250 mM NaCl, 5 mM EDTA, 50 mM NaF, 1% NP40). The loading buffer vial contains 0.5 ml 2X SDS Loading Buffer (125 mM Tris-Cl, pH6.8, 10% glycerol, 4% SDS, 0.002% Bromophenol blue, 5% beta-mercaptoethanol).
Size : 0.1 mg
Storage Instruction : Store at -80°C. Minimize freeze-thaw cycles. After addition of 2X SDS Loading Buffer, the lysates can be stored at -20°C. Product is guaranteed 6 months from the date of shipment.
Applications : ELISA, WB, IP. WB: Mix equal volume of lysates with 2X SDS Loading Buffer. Boil the mixture for 10 min before loading (for membrane protein lysates, incubate the mixture at room temperature for 30 min). Load 5 ug lysate per lane.
Gene Name : PSMD2 proteasome (prosome, macropain) 26S subunit, non-ATPase, 2 [ Homo sapiens ]
Official Symbol : PSMD2
Synonyms : PSMD2; proteasome (prosome, macropain) 26S subunit, non-ATPase, 2; 26S proteasome non-ATPase regulatory subunit 2; MGC14274; P97; Rpn1; S2; TRAP2; 55.11 protein; protein 55.11; TNFR-associated protein 2; 26S proteasome subunit p97; 26S proteasome regulatory subunit S2; 26S proteasome regulatory subunit RPN1; tumor necrosis factor receptor-associated protein 2; tumor necrosis factor type 1 receptor-associated protein 2;
Gene ID : 5708
mRNA Refseq : NM_002808
Protein Refseq : NP_002799
MIM : 606223
UniProt ID : Q13200
Chromosome Location : 3q27.3
Pathway : APC/C-mediated degradation of cell cycle proteins, organism-specific biosystem; APC/C:Cdc20 mediated degradation of Securin, organism-specific biosystem; APC/C:Cdc20 mediated degradation of mitotic proteins, organism-specific biosystem; APC/C:Cdh1 mediated degradation of Cdc20 and other APC/C:Cdh1 targeted proteins in late mitosis/early G1, organism-specific biosystem; Activation of APC/C and APC/C:Cdc20 mediated degradation of mitotic proteins, organism-specific biosystem; Activation of NF-kappaB in B Cells, organism-specific biosystem; Adaptive Immune System, organism-specific biosystem;
Function : enzyme regulator activity; protein binding;

For Research Use Only. Not intended for any clinical use. No products from Creative BioMart may be resold, modified for resale or used to manufacture commercial products without prior written approval from Creative BioMart.

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Q&As (7)

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How does PSMD2 contribute to cellular homeostasis? 10/05/2020

PSMD2 helps maintain cellular homeostasis by regulating the turnover of proteins, controlling their abundance and activity.

Can PSMD2 be a target for therapeutic interventions? 01/31/2020

Targeting components of the ubiquitin-proteasome system, including PSMD2, is explored for potential therapeutic interventions in certain diseases.

What is the primary function of PSMD2? 03/05/2019

PSMD2 is a subunit of the 26S proteasome, a cellular complex involved in protein degradation.

Are there diseases associated with PSMD2 dysfunction? 03/01/2018

Dysregulation of proteasomal function, including PSMD2, is implicated in various diseases, such as neurodegenerative disorders and cancer.

Is PSMD2 involved in protein quality control? 09/13/2017

Yes, PSMD2, as part of the 26S proteasome, contributes to protein quality control by degrading misfolded or damaged proteins.

In what cellular processes does PSMD2 play a role? 04/19/2017

PSMD2 plays a crucial role in the ubiquitin-proteasome system, participating in targeted protein degradation.

What is the subcellular location of PSMD2? 01/04/2016

PSMD2 is typically found in the nucleus and cytoplasm, reflecting its involvement in cellular protein degradation processes.

Customer Reviews (3)

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Reviews
02/25/2021

    The product's stability over extended periods was a significant advantage for our long-term research projects.

    01/05/2021

      Consistency in its performance over time made it a reliable resource for ongoing experiments.

      06/11/2019

        The absence of adverse effects on cells or organisms underscored the product's safety and suitability for biological research.

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