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Recombinant Human PRPF6 293 Cell Lysate

Cat.No. : PRPF6-2823HCL
  • Specification
  • Gene Information
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Description : Antigen standard for PRP6 pre-mRNA processing factor 6 homolog (S. cerevisiae) (PRPF6) is a lysate prepared from HEK293T cells transiently transfected with a TrueORF gene-carrying pCMV plasmid and then lysed in RIPA Buffer. Protein concentration was determined using a colorimetric assay. The antigen control carries a C-terminal Myc/DDK tag for detection.
Source : HEK 293 cells
Species : Human
Components : This product includes 3 vials: 1 vial of gene-specific cell lysate, 1 vial of control vector cell lysate, and 1 vial of loading buffer. Each lysate vial contains 0.1 mg lysate in 0.1 ml (1 mg/ml) of RIPA Buffer (50 mM Tris-HCl pH7.5, 250 mM NaCl, 5 mM EDTA, 50 mM NaF, 1% NP40). The loading buffer vial contains 0.5 ml 2X SDS Loading Buffer (125 mM Tris-Cl, pH6.8, 10% glycerol, 4% SDS, 0.002% Bromophenol blue, 5% beta-mercaptoethanol).
Size : 0.1 mg
Storage Instruction : Store at -80°C. Minimize freeze-thaw cycles. After addition of 2X SDS Loading Buffer, the lysates can be stored at -20°C. Product is guaranteed 6 months from the date of shipment.
Applications : ELISA, WB, IP. WB: Mix equal volume of lysates with 2X SDS Loading Buffer. Boil the mixture for 10 min before loading (for membrane protein lysates, incubate the mixture at room temperature for 30 min). Load 5 ug lysate per lane.
Gene Name : PRPF6 PRP6 pre-mRNA processing factor 6 homolog (S. cerevisiae) [ Homo sapiens ]
Official Symbol : PRPF6
Synonyms : PRPF6; PRP6 pre-mRNA processing factor 6 homolog (S. cerevisiae); C20orf14, chromosome 20 open reading frame 14 , PRP6 pre mRNA processing factor 6 homolog (yeast); pre-mRNA-processing factor 6; ANT 1; bB152O15.1; Prp6; TOM; U5 102K; PRP6 homolog; U5-102 kDa protein; U5 snRNP-associated 102 kDa protein; androgen receptor N-terminal domain transactivating protein-1; androgen receptor N-terminal domain-transactivating protein 1; putative mitochondrial outer membrane protein import receptor; p102 U5 small nuclear ribonucleoprotein particle-binding protein; ANT1; ANT-1; hPrp6; U5-102K; C20orf14;
Gene ID : 24148
mRNA Refseq : NM_012469
Protein Refseq : NP_036601
MIM : 613979
UniProt ID : O94906
Chromosome Location : 20q13.33
Pathway : Gene Expression, organism-specific biosystem; Processing of Capped Intron-Containing Pre-mRNA, organism-specific biosystem; Spliceosome, organism-specific biosystem; Spliceosome, conserved biosystem; Spliceosome, U4/U6.U5 tri-snRNP, organism-specific biosystem; mRNA Splicing, organism-specific biosystem; mRNA Splicing - Major Pathway, organism-specific biosystem;
Function : androgen receptor binding; protein binding; ribonucleoprotein complex binding; transcription coactivator activity;

For Research Use Only. Not intended for any clinical use. No products from Creative BioMart may be resold, modified for resale or used to manufacture commercial products without prior written approval from Creative BioMart.

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