"PRPF4B" Related Products

Recombinant Human PRPF4B 293 Cell Lysate

Cat.No.: PRPF4B-2824HCL
Description: Antigen standard for PRP4 pre-mRNA processing factor 4 homolog B (yeast) (PRPF4B) is a lysate prepared from HEK293T cells transiently transfected with a TrueORF gene-carrying pCMV plasmid and then lysed in RIPA Buffer. Protein concentration was determined using a colorimetric assay. The antigen control carries a C-terminal Myc/DDK tag for detection.
Source: HEK 293 cells
Species: Human
Components: This product includes 3 vials: 1 vial of gene-specific cell lysate, 1 vial of control vector cell lysate, and 1 vial of loading buffer. Each lysate vial contains 0.1 mg lysate in 0.1 ml (1 mg/ml) of RIPA Buffer (50 mM Tris-HCl pH7.5, 250 mM NaCl, 5 mM EDTA, 50 mM NaF, 1% NP40). The loading buffer vial contains 0.5 ml 2X SDS Loading Buffer (125 mM Tris-Cl, pH6.8, 10% glycerol, 4% SDS, 0.002% Bromophenol blue, 5% beta-mercaptoethanol).
Size: 0.1 mg
Storage Instruction: Store at -80°C. Minimize freeze-thaw cycles. After addition of 2X SDS Loading Buffer, the lysates can be stored at -20°C. Product is guaranteed 6 months from the date of shipment.
Applications: ELISA, WB, IP. WB: Mix equal volume of lysates with 2X SDS Loading Buffer. Boil the mixture for 10 min before loading (for membrane protein lysates, incubate the mixture at room temperature for 30 min). Load 5 ug lysate per lane.
Gene Name: PRPF4B PRP4 pre-mRNA processing factor 4 homolog B (yeast) [ Homo sapiens ]
Official Symbol: PRPF4B
Synonyms: PRPF4B; PRP4 pre-mRNA processing factor 4 homolog B (yeast); serine/threonine-protein kinase PRP4 homolog; KIAA0536; PR4H; Prp4; PRP4 kinase; protein-serine/threonine kinase; PRP4 pre-mRNA-processing factor 4 homolog; dJ1013A10.1 (PRP4 protein kinase homolog); PRP4; PRP4H; PRP4K; dJ1013A10.1;
Gene ID: 8899
mRNA Refseq: NM_003913
Protein Refseq: NP_003904
MIM: 602338
UniProt ID: Q13523
Chromosome Location: 6p24.2
Pathway: mRNA processing, organism-specific biosystem;
Function: ATP binding; nucleotide binding; protein binding; protein kinase activity; protein serine/threonine kinase activity;

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