"PRKG2" Related Products

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Recombinant Human PRKG2 293 Cell Lysate

Cat.No.: PRKG2-2849HCL
Description: Antigen standard for protein kinase, cGMP-dependent, type II (PRKG2) is a lysate prepared from HEK293T cells transiently transfected with a TrueORF gene-carrying pCMV plasmid and then lysed in RIPA Buffer. Protein concentration was determined using a colorimetric assay. The antigen control carries a C-terminal Myc/DDK tag for detection.
Source: HEK 293 cells
Species: Human
Components: This product includes 3 vials: 1 vial of gene-specific cell lysate, 1 vial of control vector cell lysate, and 1 vial of loading buffer. Each lysate vial contains 0.1 mg lysate in 0.1 ml (1 mg/ml) of RIPA Buffer (50 mM Tris-HCl pH7.5, 250 mM NaCl, 5 mM EDTA, 50 mM NaF, 1% NP40). The loading buffer vial contains 0.5 ml 2X SDS Loading Buffer (125 mM Tris-Cl, pH6.8, 10% glycerol, 4% SDS, 0.002% Bromophenol blue, 5% beta-mercaptoethanol).
Size: 0.1 mg
Storage Instruction: Store at -80°C. Minimize freeze-thaw cycles. After addition of 2X SDS Loading Buffer, the lysates can be stored at -20°C. Product is guaranteed 6 months from the date of shipment.
Applications: ELISA, WB, IP. WB: Mix equal volume of lysates with 2X SDS Loading Buffer. Boil the mixture for 10 min before loading (for membrane protein lysates, incubate the mixture at room temperature for 30 min). Load 5 ug lysate per lane.
Gene Name: PRKG2 protein kinase, cGMP-dependent, type II [ Homo sapiens ]
Official Symbol: PRKG2
Synonyms: PRKG2; protein kinase, cGMP-dependent, type II; cGMP-dependent protein kinase 2; cGKII; PRKGR2; cGK2; cGK 2; cGMP-dependent protein kinase II;
Gene ID: 5593
mRNA Refseq: NM_006259
Protein Refseq: NP_006250
MIM: 601591
UniProt ID: Q13237
Chromosome Location: 4q13.1-q21.1
Pathway: Gap junction, organism-specific biosystem; Gap junction, conserved biosystem; Hemostasis, organism-specific biosystem; Long-term depression, organism-specific biosystem; Long-term depression, conserved biosystem; Metabolism, organism-specific biosystem; Metabolism of nitric oxide, organism-specific biosystem;
Function: ATP binding; cGMP binding; cGMP-dependent protein kinase activity; nucleotide binding; protein kinase activity; protein serine/threonine kinase activity;

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