"PRKCB" Related Products


Recombinant Human PRKCB 293 Cell Lysate

Cat.No.: PRKCB-2859HCL
Description: Antigen standard for protein kinase C, beta (PRKCB), transcript variant 2 is a lysate prepared from HEK293T cells transiently transfected with a TrueORF gene-carrying pCMV plasmid and then lysed in RIPA Buffer. Protein concentration was determined using a colorimetric assay. The antigen control carries a C-terminal Myc/DDK tag for detection.
Source: HEK 293 cells
Species: Human
Components: This product includes 3 vials: 1 vial of gene-specific cell lysate, 1 vial of control vector cell lysate, and 1 vial of loading buffer. Each lysate vial contains 0.1 mg lysate in 0.1 ml (1 mg/ml) of RIPA Buffer (50 mM Tris-HCl pH7.5, 250 mM NaCl, 5 mM EDTA, 50 mM NaF, 1% NP40). The loading buffer vial contains 0.5 ml 2X SDS Loading Buffer (125 mM Tris-Cl, pH6.8, 10% glycerol, 4% SDS, 0.002% Bromophenol blue, 5% beta-mercaptoethanol).
Size: 0.1 mg
Storage Instruction: Store at -80°C. Minimize freeze-thaw cycles. After addition of 2X SDS Loading Buffer, the lysates can be stored at -20°C. Product is guaranteed 6 months from the date of shipment.
Applications: ELISA, WB, IP. WB: Mix equal volume of lysates with 2X SDS Loading Buffer. Boil the mixture for 10 min before loading (for membrane protein lysates, incubate the mixture at room temperature for 30 min). Load 5 ug lysate per lane.
Gene Name: PRKCB protein kinase C, beta [ Homo sapiens ]
Official Symbol: PRKCB
Synonyms: PRKCB; protein kinase C, beta; PKCB, PRKCB1, PRKCB2, protein kinase C, beta 1; protein kinase C beta type; PKC-B; protein kinase C, beta 1 polypeptide; PKCB; PRKCB1; PRKCB2; PKC-beta; MGC41878;
Gene ID: 5579
mRNA Refseq: NM_002738
Protein Refseq: NP_002729
MIM: 176970
UniProt ID: P05771
Chromosome Location: 16p12
Pathway: Activation of NF-kappaB in B Cells, organism-specific biosystem; Adaptive Immune System, organism-specific biosystem; African trypanosomiasis, organism-specific biosystem; African trypanosomiasis, conserved biosystem; Aldosterone-regulated sodium reabsorption, organism-specific biosystem; Aldosterone-regulated sodium reabsorption, conserved biosystem; Amoebiasis, organism-specific biosystem;
Function: ATP binding; androgen receptor binding; chromatin binding; histone binding; histone kinase activity (H3-T6 specific); ligand-dependent nuclear receptor transcription coactivator activity; metal ion binding; nucleotide binding; protein binding; protein kinase C activity; protein kinase C binding; zinc ion binding;

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