Recombinant Human PRKAR2A 293 Cell Lysate

• Cat.No.: PRKAR2A-2861HCL

Specification

• Description: Antigen standard for protein kinase, cAMP-dependent, regulatory, type II, alpha (PRKAR2A) is a lysate prepared from HEK293T cells transiently transfected with a TrueORF gene-carrying pCMV plasmid and then lysed in RIPA Buffer. Protein concentration was determined using a colorimetric assay. The antigen control carries a C-terminal Myc/DDK tag for detection.
• Source: HEK 293 cells
• Species: Human
• Components: This product includes 3 vials: 1 vial of gene-specific cell lysate, 1 vial of control vector cell lysate, and 1 vial of loading buffer. Each lysate vial contains 0.1 mg lysate in 0.1 ml (1 mg/ml) of RIPA Buffer (50 mM Tris-HCl pH7.5, 250 mM NaCl, 5 mM EDTA, 50 mM NaF, 1% NP40). The loading buffer vial contains 0.5 ml 2X SDS Loading Buffer (125 mM Tris-Cl, pH6.8, 10% glycerol, 4% SDS, 0.002% Bromophenol blue, 5% beta-mercaptoethanol).
• Size: 0.1 mg
• Storage Instruction: Store at -80°C. Minimize freeze-thaw cycles. After addition of 2X SDS Loading Buffer, the lysates can be stored at -20°C. Product is guaranteed 6 months from the date of shipment.
• Applications: ELISA, WB, IP. WB: Mix equal volume of lysates with 2X SDS Loading Buffer. Boil the mixture for 10 min before loading (for membrane protein lysates, incubate the mixture at room temperature for 30 min). Load 5 ug lysate per lane.

Gene Information

• Gene Name: PRKAR2A protein kinase, cAMP-dependent, regulatory, type II, alpha [ Homo sapiens ]
• Official Symbol: PRKAR2A
• Synonyms: PRKAR2A; protein kinase, cAMP-dependent, regulatory, type II, alpha; PRKAR2; cAMP-dependent protein kinase type II-alpha regulatory subunit; protein kinase A, RII-alpha subunit; cAMP-dependent protein kinase regulatory subunit RII alpha; PKR2; MGC3606
• Gene ID: 5576
• mRNA Refseq: NM_004157
• Protein Refseq: NP_004148
• MIM: 176910
• UniProt ID: P13861
• Chromosome Location: 3p21.3-p21.2
• Pathway: Apoptosis, organism-specific biosystem; Apoptosis, conserved biosystem; Aquaporin-mediated transport, organism-specific biosystem; Ca-dependent events, organism-specific biosystem; CaM pathway, organism-specific biosystem; Calcium Regulation in the Cardiac Cell, organism-specific biosystem; Calmodulin induced events, organism-specific biosystem
• Function: cAMP binding; cAMP-dependent protein kinase regulator activity; cAMP-dependent protein kinase regulator activity; kinase activity; nucleotide binding; protein domain specific binding; protein homodimerization activity; ubiquitin protein ligase binding