"PRDX3" Related Products


Recombinant Human PRDX3 293 Cell Lysate

Cat.No. : PRDX3-2881HCL
Description : Antigen standard for peroxiredoxin 3 (PRDX3), nuclear gene encoding mitochondrial protein, transcript variant 1 is a lysate prepared from HEK293T cells transiently transfected with a TrueORF gene-carrying pCMV plasmid and then lysed in RIPA Buffer. Protein concentration was determined using a colorimetric assay. The antigen control carries a C-terminal Myc/DDK tag for detection.
Source : HEK 293 cells
Species : Human
Components : This product includes 3 vials: 1 vial of gene-specific cell lysate, 1 vial of control vector cell lysate, and 1 vial of loading buffer. Each lysate vial contains 0.1 mg lysate in 0.1 ml (1 mg/ml) of RIPA Buffer (50 mM Tris-HCl pH7.5, 250 mM NaCl, 5 mM EDTA, 50 mM NaF, 1% NP40). The loading buffer vial contains 0.5 ml 2X SDS Loading Buffer (125 mM Tris-Cl, pH6.8, 10% glycerol, 4% SDS, 0.002% Bromophenol blue, 5% beta-mercaptoethanol).
Size : 0.1 mg
Storage Instruction : Store at -80°C. Minimize freeze-thaw cycles. After addition of 2X SDS Loading Buffer, the lysates can be stored at -20°C. Product is guaranteed 6 months from the date of shipment.
Applications : ELISA, WB, IP. WB: Mix equal volume of lysates with 2X SDS Loading Buffer. Boil the mixture for 10 min before loading (for membrane protein lysates, incubate the mixture at room temperature for 30 min). Load 5 ug lysate per lane.
Gene Name : PRDX3 peroxiredoxin 3 [ Homo sapiens ]
Official Symbol : PRDX3
Synonyms : PRDX3; peroxiredoxin 3; antioxidant protein 1 , AOP1; thioredoxin-dependent peroxide reductase, mitochondrial; AOP 1; MER5; SP 22; peroxiredoxin-3; peroxiredoxin III; protein MER5 homolog; antioxidant protein 1; AOP1; AOP-1; SP-22; HBC189; PRO1748; prx-III; MGC24293; MGC104387;
Gene ID : 10935
mRNA Refseq : NM_006793
Protein Refseq : NP_006784
MIM : 604769
UniProt ID : P30048
Chromosome Location : 10q25-q26
Pathway : Selenium Pathway, organism-specific biosystem; Validated targets of C-MYC transcriptional activation, organism-specific biosystem;
Function : alkyl hydroperoxide reductase activity; cysteine-type endopeptidase inhibitor activity involved in apoptotic process; identical protein binding; kinase binding; oxidoreductase activity; peroxidase activity; peroxiredoxin activity; protein C-terminus binding; protein binding; protein kinase binding;

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