"PPP4R1" Related Products

Recombinant Human PPP4R1 293 Cell Lysate

Cat.No.: PPP4R1-2911HCL
Description: Antigen standard for protein phosphatase 4, regulatory subunit 1 (PPP4R1), transcript variant 2 is a lysate prepared from HEK293T cells transiently transfected with a TrueORF gene-carrying pCMV plasmid and then lysed in RIPA Buffer. Protein concentration was determined using a colorimetric assay. The antigen control carries a C-terminal Myc/DDK tag for detection.
Source: HEK 293 cells
Species: Human
Components: This product includes 3 vials: 1 vial of gene-specific cell lysate, 1 vial of control vector cell lysate, and 1 vial of loading buffer. Each lysate vial contains 0.1 mg lysate in 0.1 ml (1 mg/ml) of RIPA Buffer (50 mM Tris-HCl pH7.5, 250 mM NaCl, 5 mM EDTA, 50 mM NaF, 1% NP40). The loading buffer vial contains 0.5 ml 2X SDS Loading Buffer (125 mM Tris-Cl, pH6.8, 10% glycerol, 4% SDS, 0.002% Bromophenol blue, 5% beta-mercaptoethanol).
Size: 0.1 mg
Storage Instruction: Store at -80°C. Minimize freeze-thaw cycles. After addition of 2X SDS Loading Buffer, the lysates can be stored at -20°C. Product is guaranteed 6 months from the date of shipment.
Applications: ELISA, WB, IP. WB: Mix equal volume of lysates with 2X SDS Loading Buffer. Boil the mixture for 10 min before loading (for membrane protein lysates, incubate the mixture at room temperature for 30 min). Load 5 ug lysate per lane.
Gene Name: PPP4R1 protein phosphatase 4, regulatory subunit 1 [ Homo sapiens ]
Official Symbol: PPP4R1
Synonyms: PPP4R1; protein phosphatase 4, regulatory subunit 1; serine/threonine-protein phosphatase 4 regulatory subunit 1; PP4R1; serine/threonine phosphatase 4; PP4(Rmeg);
Gene ID: 9989
mRNA Refseq: NM_001042388
Protein Refseq: NP_001035847
MIM: 604908
UniProt ID: Q8TF05
Chromosome Location: 18p11.22
Function: contributes_to phosphoprotein phosphatase activity; protein binding; protein phosphatase type 4 regulator activity;

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