Recombinant Human PPP1CB 293 Cell Lysate

• Cat.No.: PPP1CB-2949HCL

Specification

• Description: Antigen standard for protein phosphatase 1, catalytic subunit, beta isoform (PPP1CB), transcript variant 1 is a lysate prepared from HEK293T cells transiently transfected with a TrueORF gene-carrying pCMV plasmid and then lysed in RIPA Buffer. Protein concentration was determined using a colorimetric assay. The antigen control carries a C-terminal Myc/DDK tag for detection.
• Source: HEK 293 cells
• Species: Human
• Components: This product includes 3 vials: 1 vial of gene-specific cell lysate, 1 vial of control vector cell lysate, and 1 vial of loading buffer. Each lysate vial contains 0.1 mg lysate in 0.1 ml (1 mg/ml) of RIPA Buffer (50 mM Tris-HCl pH7.5, 250 mM NaCl, 5 mM EDTA, 50 mM NaF, 1% NP40). The loading buffer vial contains 0.5 ml 2X SDS Loading Buffer (125 mM Tris-Cl, pH6.8, 10% glycerol, 4% SDS, 0.002% Bromophenol blue, 5% beta-mercaptoethanol).
• Size: 0.1 mg
• Storage Instruction: Store at -80°C. Minimize freeze-thaw cycles. After addition of 2X SDS Loading Buffer, the lysates can be stored at -20°C. Product is guaranteed 6 months from the date of shipment.
• Applications: ELISA, WB, IP. WB: Mix equal volume of lysates with 2X SDS Loading Buffer. Boil the mixture for 10 min before loading (for membrane protein lysates, incubate the mixture at room temperature for 30 min). Load 5 ug lysate per lane.

Gene Information

• Gene Name: PPP1CB protein phosphatase 1, catalytic subunit, beta isozyme [ Homo sapiens ]
• Official Symbol: PPP1CB
• Synonyms: PPP1CB; protein phosphatase 1, catalytic subunit, beta isozyme; protein phosphatase 1, catalytic subunit, beta isoform; serine/threonine-protein phosphatase PP1-beta catalytic subunit; PP 1B; PP1beta; protein phosphatase 1-beta; protein phosphatase 1-delta; protein phosphatase 1, catalytic subunit, delta isoform; serine/threonine protein phosphatase PP1-beta catalytic subunit; PP-1B; PPP1CD; MGC3672
• Gene ID: 5500
• mRNA Refseq: NM_002709
• Protein Refseq: NP_002700
• MIM: 600590
• UniProt ID: P62140
• Chromosome Location: 2p23
• Pathway: Amphetamine addiction, organism-specific biosystem; Amphetamine addiction, conserved biosystem; Dopaminergic synapse, organism-specific biosystem; Dopaminergic synapse, conserved biosystem; Focal adhesion, organism-specific biosystem; Focal adhesion, conserved biosystem; G13 Signaling Pathway, organism-specific biosystem
• Function: hydrolase activity; metal ion binding; myosin phosphatase activity; myosin-light-chain-phosphatase activity; protein binding; protein kinase binding