Recombinant Human PPM1A 293 Cell Lysate

• Cat.No.: PPM1A-2964HCL

Specification

• Description: Antigen standard for protein phosphatase 1A (formerly 2C), magnesium-dependent, alpha isoform (PPM1A), transcript variant 1 is a lysate prepared from HEK293T cells transiently transfected with a TrueORF gene-carrying pCMV plasmid and then lysed in RIPA Buffer. Protein concentration was determined using a colorimetric assay. The antigen control carries a C-terminal Myc/DDK tag for detection.
• Source: HEK 293 cells
• Species: Human
• Components: This product includes 3 vials: 1 vial of gene-specific cell lysate, 1 vial of control vector cell lysate, and 1 vial of loading buffer. Each lysate vial contains 0.1 mg lysate in 0.1 ml (1 mg/ml) of RIPA Buffer (50 mM Tris-HCl pH7.5, 250 mM NaCl, 5 mM EDTA, 50 mM NaF, 1% NP40). The loading buffer vial contains 0.5 ml 2X SDS Loading Buffer (125 mM Tris-Cl, pH6.8, 10% glycerol, 4% SDS, 0.002% Bromophenol blue, 5% beta-mercaptoethanol).
• Size: 0.1 mg
• Storage Instruction: Store at -80°C. Minimize freeze-thaw cycles. After addition of 2X SDS Loading Buffer, the lysates can be stored at -20°C. Product is guaranteed 6 months from the date of shipment.
• Applications: ELISA, WB, IP. WB: Mix equal volume of lysates with 2X SDS Loading Buffer. Boil the mixture for 10 min before loading (for membrane protein lysates, incubate the mixture at room temperature for 30 min). Load 5 ug lysate per lane.

Gene Information

• Gene Name: PPM1A protein phosphatase, Mg2+/Mn2+ dependent, 1A [ Homo sapiens ]
• Official Symbol: PPM1A
• Synonyms: PPM1A; protein phosphatase, Mg2+/Mn2+ dependent, 1A; protein phosphatase 1A (formerly 2C), magnesium dependent, alpha isoform; protein phosphatase 1A; MGC9201; phosphatase 2C alpha; PP2CA; PP2Calpha; protein phosphatase 2C; alpha isoform; protein phosphatase 1A (formerly 2C), magnesium-dependent, alpha isoform; PP2C-ALPHA; FLJ42306
• Gene ID: 5494
• mRNA Refseq: NM_021003
• Protein Refseq: NP_066283
• MIM: 606108
• UniProt ID: P35813
• Chromosome Location: 14q23.1
• Pathway: BMP receptor signaling, organism-specific biosystem; Energy dependent regulation of mTOR by LKB1-AMPK, organism-specific biosystem; IRS-mediated signalling, organism-specific biosystem; IRS-related events, organism-specific biosystem; Insulin receptor signalling cascade, organism-specific biosystem; MAPK signaling pathway, organism-specific biosystem; MAPK signaling pathway, organism-specific biosystem
• Function: R-SMAD binding; calmodulin-dependent protein phosphatase activity; hydrolase activity; magnesium ion binding; manganese ion binding; protein C-terminus binding; protein binding; protein serine/threonine phosphatase activity; signal transducer activity