"PPIE" Related Products


Recombinant Human PPIE 293 Cell Lysate

Cat.No.: PPIE-2971HCL
Description: Antigen standard for peptidylprolyl isomerase E (cyclophilin E) (PPIE), transcript variant 1 is a lysate prepared from HEK293T cells transiently transfected with a TrueORF gene-carrying pCMV plasmid and then lysed in RIPA Buffer. Protein concentration was determined using a colorimetric assay. The antigen control carries a C-terminal Myc/DDK tag for detection.
Source: HEK 293 cells
Species: Human
Components: This product includes 3 vials: 1 vial of gene-specific cell lysate, 1 vial of control vector cell lysate, and 1 vial of loading buffer. Each lysate vial contains 0.1 mg lysate in 0.1 ml (1 mg/ml) of RIPA Buffer (50 mM Tris-HCl pH7.5, 250 mM NaCl, 5 mM EDTA, 50 mM NaF, 1% NP40). The loading buffer vial contains 0.5 ml 2X SDS Loading Buffer (125 mM Tris-Cl, pH6.8, 10% glycerol, 4% SDS, 0.002% Bromophenol blue, 5% beta-mercaptoethanol).
Size: 0.1 mg
Storage Instruction: Store at -80°C. Minimize freeze-thaw cycles. After addition of 2X SDS Loading Buffer, the lysates can be stored at -20°C. Product is guaranteed 6 months from the date of shipment.
Applications: ELISA, WB, IP. WB: Mix equal volume of lysates with 2X SDS Loading Buffer. Boil the mixture for 10 min before loading (for membrane protein lysates, incubate the mixture at room temperature for 30 min). Load 5 ug lysate per lane.
Gene Name: PPIE peptidylprolyl isomerase E (cyclophilin E) [ Homo sapiens ]
Official Symbol: PPIE
Synonyms: PPIE; peptidylprolyl isomerase E (cyclophilin E); peptidyl-prolyl cis-trans isomerase E; cyclophilin 33; cyclophilin E; CyP 33; MGC3736; MGC111222; peptidyl prolyl cis trans isomerase E; peptidylprolyl isomerase E; isoform 1; PPIase E; rotamase E; cyclophilin-33; CYP33; CYP-33;
Gene ID: 10450
mRNA Refseq: NM_001195007
Protein Refseq: NP_001181936
MIM: 602435
UniProt ID: Q9UNP9
Chromosome Location: 1p32
Pathway: Spliceosome, organism-specific biosystem; Spliceosome, conserved biosystem; Spliceosome, 35S U5-snRNP, organism-specific biosystem;
Function: RNA binding; RNA binding; cyclosporin A binding; isomerase activity; nucleotide binding; peptidyl-prolyl cis-trans isomerase activity; peptidyl-prolyl cis-trans isomerase activity; protein binding;

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