"PPID" Related Products


Recombinant Human PPID 293 Cell Lysate

Cat.No.: PPID-2972HCL
Description: Antigen standard for peptidylprolyl isomerase D (PPID) is a lysate prepared from HEK293T cells transiently transfected with a TrueORF gene-carrying pCMV plasmid and then lysed in RIPA Buffer. Protein concentration was determined using a colorimetric assay. The antigen control carries a C-terminal Myc/DDK tag for detection.
Source: HEK 293 cells
Species: Human
Components: This product includes 3 vials: 1 vial of gene-specific cell lysate, 1 vial of control vector cell lysate, and 1 vial of loading buffer. Each lysate vial contains 0.1 mg lysate in 0.1 ml (1 mg/ml) of RIPA Buffer (50 mM Tris-HCl pH7.5, 250 mM NaCl, 5 mM EDTA, 50 mM NaF, 1% NP40). The loading buffer vial contains 0.5 ml 2X SDS Loading Buffer (125 mM Tris-Cl, pH6.8, 10% glycerol, 4% SDS, 0.002% Bromophenol blue, 5% beta-mercaptoethanol).
Size: 0.1 mg
Storage Instruction: Store at -80°C. Minimize freeze-thaw cycles. After addition of 2X SDS Loading Buffer, the lysates can be stored at -20°C. Product is guaranteed 6 months from the date of shipment.
Applications: ELISA, WB, IP. WB: Mix equal volume of lysates with 2X SDS Loading Buffer. Boil the mixture for 10 min before loading (for membrane protein lysates, incubate the mixture at room temperature for 30 min). Load 5 ug lysate per lane.
Gene Name: PPID peptidylprolyl isomerase D [ Homo sapiens ]
Official Symbol: PPID
Synonyms: PPID; peptidylprolyl isomerase D; peptidylprolyl isomerase D (cyclophilin D); peptidyl-prolyl cis-trans isomerase D; cyclophilin 40; CYP 40; PPIase D; rotamase D; cyclophilin D; cyclophilin-40; cyclophilin-related protein; 40 kDa peptidyl-prolyl cis-trans isomerase D; CYPD; CYP-40; MGC33096;
Gene ID: 5481
mRNA Refseq: NM_005038
Protein Refseq: NP_005029
MIM: 601753
UniProt ID: Q08752
Chromosome Location: 4q31.3
Pathway: C-MYB transcription factor network, organism-specific biosystem;
Function: cyclosporin A binding; heat shock protein binding; isomerase activity; peptidyl-prolyl cis-trans isomerase activity; protein binding;

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