"PPAN" Related Products

Recombinant Human PPAN 293 Cell Lysate

Cat.No.: PPAN-2993HCL
Description: Antigen standard for peter pan homolog (Drosophila) (PPAN) is a lysate prepared from HEK293T cells transiently transfected with a TrueORF gene-carrying pCMV plasmid and then lysed in RIPA Buffer. Protein concentration was determined using a colorimetric assay. The antigen control carries a C-terminal Myc/DDK tag for detection.
Source: HEK 293 cells
Species: Human
Components: This product includes 3 vials: 1 vial of gene-specific cell lysate, 1 vial of control vector cell lysate, and 1 vial of loading buffer. Each lysate vial contains 0.1 mg lysate in 0.1 ml (1 mg/ml) of RIPA Buffer (50 mM Tris-HCl pH7.5, 250 mM NaCl, 5 mM EDTA, 50 mM NaF, 1% NP40). The loading buffer vial contains 0.5 ml 2X SDS Loading Buffer (125 mM Tris-Cl, pH6.8, 10% glycerol, 4% SDS, 0.002% Bromophenol blue, 5% beta-mercaptoethanol).
Size: 0.1 mg
Storage Instruction: Store at -80°C. Minimize freeze-thaw cycles. After addition of 2X SDS Loading Buffer, the lysates can be stored at -20°C. Product is guaranteed 6 months from the date of shipment.
Applications: ELISA, WB, IP. WB: Mix equal volume of lysates with 2X SDS Loading Buffer. Boil the mixture for 10 min before loading (for membrane protein lysates, incubate the mixture at room temperature for 30 min). Load 5 ug lysate per lane.
Gene Name: PPAN peter pan homolog (Drosophila) [ Homo sapiens ]
Official Symbol: PPAN
Synonyms: SSF; SSF1; SSF2; BXDC3; SSF-1; suppressor of SWI4 1 homolog; suppressor of sterile four 1; homolog of S. cerevisiae SSF1; second-step splicing factor 1; brix domain-containing protein 3
Gene ID: 56342
mRNA Refseq: NM_020230
Protein Refseq: NP_064615
MIM: 607793
UniProt ID: Q9NQ55
Chromosome Location: 19p13
Pathway: Class A/1 (Rhodopsin-like receptors), organism-specific biosystem; GPCR ligand binding, organism-specific biosystem; Signal Transduction, organism-specific biosystem
Function: poly(A) RNA binding

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