"POMC" Related Products

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Recombinant Human POMC 293 Cell Lysate

Cat.No.: POMC-3018HCL
Description: Antigen standard for proopiomelanocortin (POMC), transcript variant 1 is a lysate prepared from HEK293T cells transiently transfected with a TrueORF gene-carrying pCMV plasmid and then lysed in RIPA Buffer. Protein concentration was determined using a colorimetric assay. The antigen control carries a C-terminal Myc/DDK tag for detection.
Source: HEK 293 cells
Species: Human
Components: This product includes 3 vials: 1 vial of gene-specific cell lysate, 1 vial of control vector cell lysate, and 1 vial of loading buffer. Each lysate vial contains 0.1 mg lysate in 0.1 ml (1 mg/ml) of RIPA Buffer (50 mM Tris-HCl pH7.5, 250 mM NaCl, 5 mM EDTA, 50 mM NaF, 1% NP40). The loading buffer vial contains 0.5 ml 2X SDS Loading Buffer (125 mM Tris-Cl, pH6.8, 10% glycerol, 4% SDS, 0.002% Bromophenol blue, 5% beta-mercaptoethanol).
Size: 0.1 mg
Storage Instruction: Store at -80°C. Minimize freeze-thaw cycles. After addition of 2X SDS Loading Buffer, the lysates can be stored at -20°C. Product is guaranteed 6 months from the date of shipment.
Applications: ELISA, WB, IP. WB: Mix equal volume of lysates with 2X SDS Loading Buffer. Boil the mixture for 10 min before loading (for membrane protein lysates, incubate the mixture at room temperature for 30 min). Load 5 ug lysate per lane.
Gene Name: POMC proopiomelanocortin [ Homo sapiens ]
Official Symbol: POMC
Synonyms: POMC; proopiomelanocortin; pro-opiomelanocortin; ACTH; adrenocorticotropic hormone; adrenocorticotropin; alpha melanocyte stimulating hormone; beta endorphin; beta lipotropin; beta melanocyte stimulating hormone; CLIP; LPH; MSH; NPP; POC; beta-LPH; beta-MSH; alpha-MSH; gamma-LPH; gamma-MSH; beta-endorphin; met-enkephalin; lipotropin beta; lipotropin gamma; melanotropin beta; melanotropin alpha; melanotropin gamma; pro-ACTH-endorphin; corticotropin-lipotropin; proopiomelanocortin preproprotein; beta-melanocyte-stimulating hormone; alpha-melanocyte-stimulating hormone; corticotropin-like intermediary peptide;
Gene ID: 5443
mRNA Refseq: NM_000939
Protein Refseq: NP_000930
MIM: 176830
UniProt ID: P01189
Chromosome Location: 2p23
Pathway: Adipocytokine signaling pathway, organism-specific biosystem; Adipocytokine signaling pathway, conserved biosystem; Androgen biosynthesis, organism-specific biosystem; Biological oxidations, organism-specific biosystem; Class A/1 (Rhodopsin-like receptors), organism-specific biosystem; Cytochrome P450 - arranged by substrate type, organism-specific biosystem; Endogenous sterols, organism-specific biosystem;
Function: G-protein coupled receptor binding; hormone activity; hormone activity; receptor binding; type 1 melanocortin receptor binding; type 1 melanocortin receptor binding; type 3 melanocortin receptor binding; type 4 melanocortin receptor binding;

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