"POLE4" Related Products

Recombinant Human POLE4 293 Cell Lysate

Cat.No.: POLE4-3048HCL
Description: Antigen standard for polymerase (DNA-directed), epsilon 4 (p12 subunit) (POLE4) is a lysate prepared from HEK293T cells transiently transfected with a TrueORF gene-carrying pCMV plasmid and then lysed in RIPA Buffer. Protein concentration was determined using a colorimetric assay. The antigen control carries a C-terminal Myc/DDK tag for detection.
Source: HEK 293 cells
Species: Human
Components: This product includes 3 vials: 1 vial of gene-specific cell lysate, 1 vial of control vector cell lysate, and 1 vial of loading buffer. Each lysate vial contains 0.1 mg lysate in 0.1 ml (1 mg/ml) of RIPA Buffer (50 mM Tris-HCl pH7.5, 250 mM NaCl, 5 mM EDTA, 50 mM NaF, 1% NP40). The loading buffer vial contains 0.5 ml 2X SDS Loading Buffer (125 mM Tris-Cl, pH6.8, 10% glycerol, 4% SDS, 0.002% Bromophenol blue, 5% beta-mercaptoethanol).
Size: 0.1 mg
Storage Instruction: Store at -80°C. Minimize freeze-thaw cycles. After addition of 2X SDS Loading Buffer, the lysates can be stored at -20°C. Product is guaranteed 6 months from the date of shipment.
Applications: ELISA, WB, IP. WB: Mix equal volume of lysates with 2X SDS Loading Buffer. Boil the mixture for 10 min before loading (for membrane protein lysates, incubate the mixture at room temperature for 30 min). Load 5 ug lysate per lane.
Gene Name: POLE4 polymerase (DNA-directed), epsilon 4, accessory subunit [ Homo sapiens ]
Official Symbol: POLE4
Synonyms: p12; YHHQ1; DNA polymerase epsilon subunit 4; DNA polymerase II subunit 4; DNA polymerase epsilon p12 subunit; DNA polymerase epsilon subunit p12; polymerase (DNA-directed), epsilon 4 (p12 subunit)
Gene ID: 56655
mRNA Refseq: NM_019896
Protein Refseq: NP_063949
MIM: 607269
UniProt ID: Q9NR33
Chromosome Location: 2p12
Pathway: Base excision repair, organism-specific biosystem; DNA polymerase epsilon complex, organism-specific biosystem
Function: DNA-directed DNA polymerase activity; protein heterodimerization activity; sequence-specific DNA binding

Online Inquiry

Note: There will be extra charge for optional service!

Please input "biomart" as verification code. Please review Creative BioMart's privacy policy for more information

Optional requirements on this protein    +Expand

Price Inquiry

Welcome! For price inquiries, please feel free to contact us through the form below. We will get back to you as soon as possible.