"PMS1" Related Products

Recombinant Human PMS1 293 Cell Lysate

Cat.No.: PMS1-3085HCL
Description: Antigen standard for PMS1 postmeiotic segregation increased 1 (S. cerevisiae) (PMS1), transcript variant 1 is a lysate prepared from HEK293T cells transiently transfected with a TrueORF gene-carrying pCMV plasmid and then lysed in RIPA Buffer. Protein concentration was determined using a colorimetric assay. The antigen control carries a C-terminal Myc/DDK tag for detection.
Source: HEK 293 cells
Species: Human
Components: This product includes 3 vials: 1 vial of gene-specific cell lysate, 1 vial of control vector cell lysate, and 1 vial of loading buffer. Each lysate vial contains 0.1 mg lysate in 0.1 ml (1 mg/ml) of RIPA Buffer (50 mM Tris-HCl pH7.5, 250 mM NaCl, 5 mM EDTA, 50 mM NaF, 1% NP40). The loading buffer vial contains 0.5 ml 2X SDS Loading Buffer (125 mM Tris-Cl, pH6.8, 10% glycerol, 4% SDS, 0.002% Bromophenol blue, 5% beta-mercaptoethanol).
Size: 0.1 mg
Storage Instruction: Store at -80°C. Minimize freeze-thaw cycles. After addition of 2X SDS Loading Buffer, the lysates can be stored at -20°C. Product is guaranteed 6 months from the date of shipment.
Applications: ELISA, WB, IP. WB: Mix equal volume of lysates with 2X SDS Loading Buffer. Boil the mixture for 10 min before loading (for membrane protein lysates, incubate the mixture at room temperature for 30 min). Load 5 ug lysate per lane.
Gene Name: PMS1 PMS1 postmeiotic segregation increased 1 (S. cerevisiae) [ Homo sapiens ]
Official Symbol: PMS1
Synonyms: PMS1; PMS1 postmeiotic segregation increased 1 (S. cerevisiae); PMSL1, postmeiotic segregation increased (S. cerevisiae) 1; PMS1 protein homolog 1; mismatch repair gene PMSL1; human homolog of yeast mutL; DNA mismatch repair protein PMS1; rhabdomyosarcoma antigen MU-RMS-40.10B; rhabdomyosarcoma antigen MU-RMS-40.10E; PMSL1; hPMS1; HNPCC3; FLJ98259; DKFZp781M0253;
Gene ID: 5378
mRNA Refseq: NM_000534
Protein Refseq: NP_000525
MIM: 600258
UniProt ID: P54277
Chromosome Location: 2q31-q33
Function: ATP binding; ATPase activity; DNA binding; mismatched DNA binding; mismatched DNA binding;
Products Types ◆ Recombinant Protein

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