"PLCB1" Related Products

Recombinant Human PLCB1 293 Cell Lysate

Cat.No.: PLCB1-3131HCL
Description: Antigen standard for phospholipase C, beta 1 (phosphoinositide-specific) (PLCB1), transcript variant 1 is a lysate prepared from HEK293T cells transiently transfected with a TrueORF gene-carrying pCMV plasmid and then lysed in RIPA Buffer. Protein concentration was determined using a colorimetric assay. The antigen control carries a C-terminal Myc/DDK tag for detection.
Source: HEK 293 cells
Species: Human
Components: This product includes 3 vials: 1 vial of gene-specific cell lysate, 1 vial of control vector cell lysate, and 1 vial of loading buffer. Each lysate vial contains 0.1 mg lysate in 0.1 ml (1 mg/ml) of RIPA Buffer (50 mM Tris-HCl pH7.5, 250 mM NaCl, 5 mM EDTA, 50 mM NaF, 1% NP40). The loading buffer vial contains 0.5 ml 2X SDS Loading Buffer (125 mM Tris-Cl, pH6.8, 10% glycerol, 4% SDS, 0.002% Bromophenol blue, 5% beta-mercaptoethanol).
Size: 0.1 mg
Storage Instruction: Store at -80°C. Minimize freeze-thaw cycles. After addition of 2X SDS Loading Buffer, the lysates can be stored at -20°C. Product is guaranteed 6 months from the date of shipment.
Applications: ELISA, WB, IP. WB: Mix equal volume of lysates with 2X SDS Loading Buffer. Boil the mixture for 10 min before loading (for membrane protein lysates, incubate the mixture at room temperature for 30 min). Load 5 ug lysate per lane.
Gene Name: PLCB1 phospholipase C, beta 1 (phosphoinositide-specific) [ Homo sapiens ]
Official Symbol: PLCB1
Synonyms: PLCB1; phospholipase C, beta 1 (phosphoinositide-specific); 1-phosphatidylinositol 4,5-bisphosphate phosphodiesterase beta-1; KIAA0581; PLC I; PLC154; PLC-beta-1; phospholipase C-I; phosphoinositidase C; phospholipase C-beta-1; inositoltrisphosphohydrolase; triphosphoinositide phosphodiesterase; phosphoinositide phospholipase C-beta-1; monophosphatidylinositol phosphodiesterase; 1-phosphatidyl-D-myo-inositol-4,5-bisphosphate; 1-phosphatidylinositol-4,5-bisphosphate phosphodiesterase beta 1; 1-phosphatidylinositol-4,5-bisphosphate phosphodiesterase beta-1; PLC-I; EIEE12; PI-PLC; PLCB1A; PLCB1B; PLC-154; FLJ45792;
Gene ID: 23236
mRNA Refseq: NM_015192
Protein Refseq: NP_056007
MIM: 607120
UniProt ID: Q9NQ66
Chromosome Location: 20p12
Pathway: Activation of Kainate Receptors upon glutamate binding, organism-specific biosystem; African trypanosomiasis, organism-specific biosystem; African trypanosomiasis, conserved biosystem; Alzheimers disease, organism-specific biosystem; Alzheimers disease, conserved biosystem; Amoebiasis, organism-specific biosystem; Amoebiasis, conserved biosystem;
Function: GTPase activator activity; calcium ion binding; calmodulin binding; enzyme binding; hydrolase activity; lamin binding; phosphatidylinositol phospholipase C activity; phosphatidylinositol phospholipase C activity; phosphatidylinositol phospholipase C activity; phosphatidylinositol phospholipase C activity; phosphatidylinositol-4,5-bisphosphate binding; phospholipase C activity; protein homodimerization activity; signal transducer activity;

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