"PLA2G4C" Related Products

Recombinant Human PLA2G4C 293 Cell Lysate

Cat.No.: PLA2G4C-3140HCL
Description: Antigen standard for phospholipase A2, group IVC (cytosolic, calcium-independent) (PLA2G4C), transcript variant 1 is a lysate prepared from HEK293T cells transiently transfected with a TrueORF gene-carrying pCMV plasmid and then lysed in RIPA Buffer. Protein concentration was determined using a colorimetric assay. The antigen control carries a C-terminal Myc/DDK tag for detection.
Source: HEK 293 cells
Species: Human
Components: This product includes 3 vials: 1 vial of gene-specific cell lysate, 1 vial of control vector cell lysate, and 1 vial of loading buffer. Each lysate vial contains 0.1 mg lysate in 0.1 ml (1 mg/ml) of RIPA Buffer (50 mM Tris-HCl pH7.5, 250 mM NaCl, 5 mM EDTA, 50 mM NaF, 1% NP40). The loading buffer vial contains 0.5 ml 2X SDS Loading Buffer (125 mM Tris-Cl, pH6.8, 10% glycerol, 4% SDS, 0.002% Bromophenol blue, 5% beta-mercaptoethanol).
Size: 0.1 mg
Storage Instruction: Store at -80°C. Minimize freeze-thaw cycles. After addition of 2X SDS Loading Buffer, the lysates can be stored at -20°C. Product is guaranteed 6 months from the date of shipment.
Applications: ELISA, WB, IP. WB: Mix equal volume of lysates with 2X SDS Loading Buffer. Boil the mixture for 10 min before loading (for membrane protein lysates, incubate the mixture at room temperature for 30 min). Load 5 ug lysate per lane.
Gene Name: PLA2G4C phospholipase A2, group IVC (cytosolic, calcium-independent) [ Homo sapiens ]
Official Symbol: PLA2G4C
Synonyms: PLA2G4C; phospholipase A2, group IVC (cytosolic, calcium-independent); cytosolic phospholipase A2 gamma; cPLA2 gamma; phospholipase A2 group IVC; CPLA2-gamma; FLJ42247; FLJ44164; DKFZp586C0423;
Gene ID: 8605
mRNA Refseq: NM_001159322
Protein Refseq: NP_001152794
MIM: 603602
UniProt ID: Q9UP65
Chromosome Location: 19q13.3
Pathway: phospholipases, organism-specific biosystem;
Function: calcium-independent phospholipase A2 activity; hydrolase activity; phospholipid binding;

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