"PLA2G10" Related Products

Recombinant Human PLA2G10 293 Cell Lysate

Cat.No.: PLA2G10-3145HCL
Description: Antigen standard for phospholipase A2, group X (PLA2G10) is a lysate prepared from HEK293T cells transiently transfected with a TrueORF gene-carrying pCMV plasmid and then lysed in RIPA Buffer. Protein concentration was determined using a colorimetric assay. The antigen control carries a C-terminal Myc/DDK tag for detection.
Source: HEK 293 cells
Species: Human
Components: This product includes 3 vials: 1 vial of gene-specific cell lysate, 1 vial of control vector cell lysate, and 1 vial of loading buffer. Each lysate vial contains 0.1 mg lysate in 0.1 ml (1 mg/ml) of RIPA Buffer (50 mM Tris-HCl pH7.5, 250 mM NaCl, 5 mM EDTA, 50 mM NaF, 1% NP40). The loading buffer vial contains 0.5 ml 2X SDS Loading Buffer (125 mM Tris-Cl, pH6.8, 10% glycerol, 4% SDS, 0.002% Bromophenol blue, 5% beta-mercaptoethanol).
Size: 0.1 mg
Storage Instruction: Store at -80°C. Minimize freeze-thaw cycles. After addition of 2X SDS Loading Buffer, the lysates can be stored at -20°C. Product is guaranteed 6 months from the date of shipment.
Applications: ELISA, WB, IP. WB: Mix equal volume of lysates with 2X SDS Loading Buffer. Boil the mixture for 10 min before loading (for membrane protein lysates, incubate the mixture at room temperature for 30 min). Load 5 ug lysate per lane.
Gene Name: PLA2G10 phospholipase A2, group X [ Homo sapiens ]
Official Symbol: PLA2G10
Synonyms: PLA2G10; phospholipase A2, group X; group 10 secretory phospholipase A2; GXPLA2; sPLA2-X; GX sPLA2; group X secretory phospholipase A2; phosphatidylcholine 2-acylhydrolase 10; SPLA2; GXSPLA2; MGC119918; MGC119919; MGC133367;
Gene ID: 8399
mRNA Refseq: NM_003561
Protein Refseq: NP_003552
MIM: 603603
UniProt ID: O15496
Chromosome Location: 16p13.1-p12
Pathway: Arachidonic acid metabolism, organism-specific biosystem; Arachidonic acid metabolism, conserved biosystem; Ether lipid metabolism, organism-specific biosystem; Ether lipid metabolism, conserved biosystem; Fat digestion and absorption, organism-specific biosystem; Fat digestion and absorption, conserved biosystem; Fc epsilon RI signaling pathway, organism-specific biosystem;
Function: calcium ion binding; hydrolase activity; phospholipase A2 activity; phospholipase activity;

Online Inquiry

Note: There will be extra charge for optional service!

Please input "biomart" as verification code. Please review Creative BioMart's privacy policy for more information

Optional requirements on this protein    +Expand

Price Inquiry

Welcome! For price inquiries, please feel free to contact us through the form below. We will get back to you as soon as possible.