Recombinant Human PKMYT1 293 Cell Lysate
Cat.No. : | PKMYT1-3151HCL |
- Specification
- Gene Information
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Description : | Antigen standard for protein kinase, membrane associated tyrosine/threonine 1 (PKMYT1), transcript variant 1 is a lysate prepared from HEK293T cells transiently transfected with a TrueORF gene-carrying pCMV plasmid and then lysed in RIPA Buffer. Protein concentration was determined using a colorimetric assay. The antigen control carries a C-terminal Myc/DDK tag for detection. |
Source : | HEK 293 cells |
Species : | Human |
Components : | This product includes 3 vials: 1 vial of gene-specific cell lysate, 1 vial of control vector cell lysate, and 1 vial of loading buffer. Each lysate vial contains 0.1 mg lysate in 0.1 ml (1 mg/ml) of RIPA Buffer (50 mM Tris-HCl pH7.5, 250 mM NaCl, 5 mM EDTA, 50 mM NaF, 1% NP40). The loading buffer vial contains 0.5 ml 2X SDS Loading Buffer (125 mM Tris-Cl, pH6.8, 10% glycerol, 4% SDS, 0.002% Bromophenol blue, 5% beta-mercaptoethanol). |
Size : | 0.1 mg |
Storage Instruction : | Store at -80°C. Minimize freeze-thaw cycles. After addition of 2X SDS Loading Buffer, the lysates can be stored at -20°C. Product is guaranteed 6 months from the date of shipment. |
Applications : | ELISA, WB, IP. WB: Mix equal volume of lysates with 2X SDS Loading Buffer. Boil the mixture for 10 min before loading (for membrane protein lysates, incubate the mixture at room temperature for 30 min). Load 5 ug lysate per lane. |
Gene Name : | PKMYT1 protein kinase, membrane associated tyrosine/threonine 1 [ Homo sapiens ] |
Official Symbol : | PKMYT1 |
Synonyms : | PKMYT1; protein kinase, membrane associated tyrosine/threonine 1; membrane-associated tyrosine- and threonine-specific cdc2-inhibitory kinase; membrane associated tyrosine and threonine specific cdc2 inhibitory kinase; MYT1; myt1 kinase; FLJ20093; DKFZp547K1610; |
Gene ID : | 9088 |
mRNA Refseq : | NM_004203 |
Protein Refseq : | NP_004194 |
MIM : | 602474 |
UniProt ID : | Q99640 |
Chromosome Location : | 16p13.3 |
Pathway : | Cell Cycle, organism-specific biosystem; Cell Cycle, Mitotic, organism-specific biosystem; Cell cycle, organism-specific biosystem; Cell cycle, organism-specific biosystem; Cell cycle, conserved biosystem; Cyclin A/B1 associated events during G2/M transition, organism-specific biosystem; Cyclin E associated events during G1/S transition, organism-specific biosystem; |
Function : | ATP binding; kinase activity; metal ion binding; nucleotide binding; protein binding; protein kinase activity; protein serine/threonine kinase activity; |
Products Types
◆ Recombinant Protein | ||
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Pkmyt1-4889M | Recombinant Mouse Pkmyt1 Protein, Myc/DDK-tagged | +Inquiry |
PKMYT1-0058H | Recombinant Human PKMYT1 Protein (L2-T499), His/GST tagged | +Inquiry |
PKMYT1-0057H | Recombinant Human PKMYT1 Protein (L2-T499), Tag Free | +Inquiry |
PKMYT1-1689H | Recombinant Human PKMYT1 Protein, His (Fc)-Avi-tagged | +Inquiry |
Related Gene
For Research Use Only. Not intended for any clinical use. No products from Creative BioMart may be resold, modified for resale or used to manufacture commercial products without prior written approval from Creative BioMart.
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Q&As (5)
Ask a questionYes, the expression levels of PKMYT1 can be indicative of the responsiveness of cancer cells to specific treatments, making it a potential predictive biomarker.
Yes, several clinical trials are investigating the efficacy of PKMYT1 inhibitors in various cancer types.
Selective PKMYT1 inhibitors aim to minimize effects on normal cells, but potential impacts on the cell cycle of non-cancerous cells are still under investigation.
Yes, understanding the individual expression levels of PKMYT1 in a patient's tumor can contribute to personalized treatment plans.
PKMYT1 regulates the cell cycle by inhibiting the activity of cyclin-dependent kinases (CDKs), particularly CDK1, which is crucial for G2/M phase transition.
Customer Reviews (3)
Write a reviewManufacturers often have a wealth of knowledge and expertise in the field of PKMYT1 protein research.
The manufacturer can assist in sourcing complementary reagents and tools, such as specific antibodies or detection systems, to complement the use of PKMYT1 protein in trials.
They may offer educational resources such as technical guides, protocols, and application notes, which can aid researchers in understanding the principles, techniques, and potential applications of PKMYT1 protein in their studies.
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