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Recombinant Human PKMYT1 293 Cell Lysate

Cat.No. : PKMYT1-3151HCL
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  • Gene Information
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Description : Antigen standard for protein kinase, membrane associated tyrosine/threonine 1 (PKMYT1), transcript variant 1 is a lysate prepared from HEK293T cells transiently transfected with a TrueORF gene-carrying pCMV plasmid and then lysed in RIPA Buffer. Protein concentration was determined using a colorimetric assay. The antigen control carries a C-terminal Myc/DDK tag for detection.
Source : HEK 293 cells
Species : Human
Components : This product includes 3 vials: 1 vial of gene-specific cell lysate, 1 vial of control vector cell lysate, and 1 vial of loading buffer. Each lysate vial contains 0.1 mg lysate in 0.1 ml (1 mg/ml) of RIPA Buffer (50 mM Tris-HCl pH7.5, 250 mM NaCl, 5 mM EDTA, 50 mM NaF, 1% NP40). The loading buffer vial contains 0.5 ml 2X SDS Loading Buffer (125 mM Tris-Cl, pH6.8, 10% glycerol, 4% SDS, 0.002% Bromophenol blue, 5% beta-mercaptoethanol).
Size : 0.1 mg
Storage Instruction : Store at -80°C. Minimize freeze-thaw cycles. After addition of 2X SDS Loading Buffer, the lysates can be stored at -20°C. Product is guaranteed 6 months from the date of shipment.
Applications : ELISA, WB, IP. WB: Mix equal volume of lysates with 2X SDS Loading Buffer. Boil the mixture for 10 min before loading (for membrane protein lysates, incubate the mixture at room temperature for 30 min). Load 5 ug lysate per lane.
Gene Name : PKMYT1 protein kinase, membrane associated tyrosine/threonine 1 [ Homo sapiens ]
Official Symbol : PKMYT1
Synonyms : PKMYT1; protein kinase, membrane associated tyrosine/threonine 1; membrane-associated tyrosine- and threonine-specific cdc2-inhibitory kinase; membrane associated tyrosine and threonine specific cdc2 inhibitory kinase; MYT1; myt1 kinase; FLJ20093; DKFZp547K1610;
Gene ID : 9088
mRNA Refseq : NM_004203
Protein Refseq : NP_004194
MIM : 602474
UniProt ID : Q99640
Chromosome Location : 16p13.3
Pathway : Cell Cycle, organism-specific biosystem; Cell Cycle, Mitotic, organism-specific biosystem; Cell cycle, organism-specific biosystem; Cell cycle, organism-specific biosystem; Cell cycle, conserved biosystem; Cyclin A/B1 associated events during G2/M transition, organism-specific biosystem; Cyclin E associated events during G1/S transition, organism-specific biosystem;
Function : ATP binding; kinase activity; metal ion binding; nucleotide binding; protein binding; protein kinase activity; protein serine/threonine kinase activity;

For Research Use Only. Not intended for any clinical use. No products from Creative BioMart may be resold, modified for resale or used to manufacture commercial products without prior written approval from Creative BioMart.

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Q&As (5)

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Can PKMYT1 be used as a predictive biomarker in cancer patients? 07/26/2022

Yes, the expression levels of PKMYT1 can be indicative of the responsiveness of cancer cells to specific treatments, making it a potential predictive biomarker.

Are there ongoing clinical trials targeting PKMYT1? 05/21/2021

Yes, several clinical trials are investigating the efficacy of PKMYT1 inhibitors in various cancer types.

How does PKMYT1 inhibition affect normal cell function? 05/07/2021

Selective PKMYT1 inhibitors aim to minimize effects on normal cells, but potential impacts on the cell cycle of non-cancerous cells are still under investigation.

Can PKMYT1 be targeted for personalized medicine in cancer treatment? 01/17/2018

Yes, understanding the individual expression levels of PKMYT1 in a patient's tumor can contribute to personalized treatment plans.

How does PKMYT1 influence cell cycle progression? 08/28/2017

PKMYT1 regulates the cell cycle by inhibiting the activity of cyclin-dependent kinases (CDKs), particularly CDK1, which is crucial for G2/M phase transition.

Customer Reviews (3)

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Reviews
01/31/2021

    Manufacturers often have a wealth of knowledge and expertise in the field of PKMYT1 protein research.

    01/22/2019

      The manufacturer can assist in sourcing complementary reagents and tools, such as specific antibodies or detection systems, to complement the use of PKMYT1 protein in trials.

      09/28/2018

        They may offer educational resources such as technical guides, protocols, and application notes, which can aid researchers in understanding the principles, techniques, and potential applications of PKMYT1 protein in their studies.

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