"PIAS3" Related Products


Recombinant Human PIAS3 293 Cell Lysate

Cat.No.: PIAS3-3202HCL
Description: Antigen standard for protein inhibitor of activated STAT, 3 (PIAS3) is a lysate prepared from HEK293T cells transiently transfected with a TrueORF gene-carrying pCMV plasmid and then lysed in RIPA Buffer. Protein concentration was determined using a colorimetric assay. The antigen control carries a C-terminal Myc/DDK tag for detection.
Source: HEK 293 cells
Species: Human
Components: This product includes 3 vials: 1 vial of gene-specific cell lysate, 1 vial of control vector cell lysate, and 1 vial of loading buffer. Each lysate vial contains 0.1 mg lysate in 0.1 ml (1 mg/ml) of RIPA Buffer (50 mM Tris-HCl pH7.5, 250 mM NaCl, 5 mM EDTA, 50 mM NaF, 1% NP40). The loading buffer vial contains 0.5 ml 2X SDS Loading Buffer (125 mM Tris-Cl, pH6.8, 10% glycerol, 4% SDS, 0.002% Bromophenol blue, 5% beta-mercaptoethanol).
Size: 0.1 mg
Storage Instruction: Store at -80°C. Minimize freeze-thaw cycles. After addition of 2X SDS Loading Buffer, the lysates can be stored at -20°C. Product is guaranteed 6 months from the date of shipment.
Applications: ELISA, WB, IP. WB: Mix equal volume of lysates with 2X SDS Loading Buffer. Boil the mixture for 10 min before loading (for membrane protein lysates, incubate the mixture at room temperature for 30 min). Load 5 ug lysate per lane.
Gene Name: PIAS3 protein inhibitor of activated STAT, 3 [ Homo sapiens ]
Official Symbol: PIAS3
Synonyms: PIAS3; protein inhibitor of activated STAT, 3; E3 SUMO-protein ligase PIAS3; FLJ14651; zinc finger; MIZ type containing 5; ZMIZ5; zinc finger, MIZ-type containing 5; protein inhibitor of activated STAT protein 3;
Gene ID: 10401
mRNA Refseq: NM_006099
Protein Refseq: NP_006090
MIM: 605987
UniProt ID: Q9Y6X2
Chromosome Location: 1q21
Pathway: Androgen Receptor Signaling Pathway, organism-specific biosystem; C-MYB transcription factor network, organism-specific biosystem; Coregulation of Androgen receptor activity, organism-specific biosystem; Hepatitis C, organism-specific biosystem; Hepatitis C, conserved biosystem; IL6-mediated signaling events, organism-specific biosystem; Jak-STAT signaling pathway, organism-specific biosystem;
Function: SUMO ligase activity; enzyme binding; ligase activity; metal ion binding; nucleic acid binding; potassium channel regulator activity; protein C-terminus binding; protein N-terminus binding; protein binding; zinc ion binding;

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