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Recombinant Human PAWR 293 Cell Lysate

Cat.No.: PAWR-3420HCL
Description: Antigen standard for PRKC, apoptosis, WT1, regulator (PAWR) is a lysate prepared from HEK293T cells transiently transfected with a TrueORF gene-carrying pCMV plasmid and then lysed in RIPA Buffer. Protein concentration was determined using a colorimetric assay. The antigen control carries a C-terminal Myc/DDK tag for detection.
Source: HEK 293 cells
Species: Human
Components: This product includes 3 vials: 1 vial of gene-specific cell lysate, 1 vial of control vector cell lysate, and 1 vial of loading buffer. Each lysate vial contains 0.1 mg lysate in 0.1 ml (1 mg/ml) of RIPA Buffer (50 mM Tris-HCl pH7.5, 250 mM NaCl, 5 mM EDTA, 50 mM NaF, 1% NP40). The loading buffer vial contains 0.5 ml 2X SDS Loading Buffer (125 mM Tris-Cl, pH6.8, 10% glycerol, 4% SDS, 0.002% Bromophenol blue, 5% beta-mercaptoethanol).
Size: 0.1 mg
Storage Instruction: Store at -80°C. Minimize freeze-thaw cycles. After addition of 2X SDS Loading Buffer, the lysates can be stored at -20°C. Product is guaranteed 6 months from the date of shipment.
Applications: ELISA, WB, IP. WB: Mix equal volume of lysates with 2X SDS Loading Buffer. Boil the mixture for 10 min before loading (for membrane protein lysates, incubate the mixture at room temperature for 30 min). Load 5 ug lysate per lane.
Gene Name: PAWR PRKC, apoptosis, WT1, regulator [ Homo sapiens ]
Official Symbol: PAWR
Synonyms: PAWR; PRKC, apoptosis, WT1, regulator; PRKC apoptosis WT1 regulator protein; par 4; PAR4; WT1-interacting protein; transcriptional repressor PAR4; prostate apoptosis response 4 protein; prostate apoptosis response protein 4; prostate apoptosis response protein PAR-4; Par-4;
Gene ID: 5074
mRNA Refseq: NM_002583
Protein Refseq: NP_002574
MIM: 601936
UniProt ID: Q96IZ0
Chromosome Location: 12q21.2
Pathway: Ceramide signaling pathway, organism-specific biosystem; Coregulation of Androgen receptor activity, organism-specific biosystem; IL-4 signaling Pathway, organism-specific biosystem;
Function: actin binding; enzyme binding; leucine zipper domain binding; protein binding; transcription corepressor activity;

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