"PATZ1" Related Products

Recombinant Human PATZ1 293 Cell Lysate

Cat.No.: PATZ1-3421HCL
Description: Antigen standard for POZ (BTB) and AT hook containing zinc finger 1 (PATZ1), transcript variant 1 is a lysate prepared from HEK293T cells transiently transfected with a TrueORF gene-carrying pCMV plasmid and then lysed in RIPA Buffer. Protein concentration was determined using a colorimetric assay. The antigen control carries a C-terminal Myc/DDK tag for detection.
Source: HEK 293 cells
Species: Human
Components: This product includes 3 vials: 1 vial of gene-specific cell lysate, 1 vial of control vector cell lysate, and 1 vial of loading buffer. Each lysate vial contains 0.1 mg lysate in 0.1 ml (1 mg/ml) of RIPA Buffer (50 mM Tris-HCl pH7.5, 250 mM NaCl, 5 mM EDTA, 50 mM NaF, 1% NP40). The loading buffer vial contains 0.5 ml 2X SDS Loading Buffer (125 mM Tris-Cl, pH6.8, 10% glycerol, 4% SDS, 0.002% Bromophenol blue, 5% beta-mercaptoethanol).
Size: 0.1 mg
Storage Instruction: Store at -80°C. Minimize freeze-thaw cycles. After addition of 2X SDS Loading Buffer, the lysates can be stored at -20°C. Product is guaranteed 6 months from the date of shipment.
Applications: ELISA, WB, IP. WB: Mix equal volume of lysates with 2X SDS Loading Buffer. Boil the mixture for 10 min before loading (for membrane protein lysates, incubate the mixture at room temperature for 30 min). Load 5 ug lysate per lane.
Gene Name: PATZ1 POZ (BTB) and AT hook containing zinc finger 1 [ Homo sapiens ]
Official Symbol: PATZ1
Synonyms: PATZ1; POZ (BTB) and AT hook containing zinc finger 1; zinc finger protein 278 , ZNF278; POZ-, AT hook-, and zinc finger-containing protein 1; dJ400N23; MAZR; PATZ; RIAZ; ZBTB19; ZSG; MAZ-related factor; zinc finger protein 278; POZ-AT hook-zinc finger protein; zinc finger sarcoma gene protein; BTB-POZ domain zinc finger transcription factor; BTB/POZ domain zinc finger transcription factor; zinc finger and BTB domain-containing protein 19; protein kinase A RI subunit alpha-associated protein; ZNF278;
Gene ID: 23598
mRNA Refseq: NM_014323
Protein Refseq: NP_055138
MIM: 605165
UniProt ID: Q9HBE1
Chromosome Location: 22q12.2
Pathway: Androgen Receptor Signaling Pathway, organism-specific biosystem; Coregulation of Androgen receptor activity, organism-specific biosystem;
Function: DNA binding; chromatin binding; metal ion binding; zinc ion binding;

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