||Recombinant Human JNK2 produced inE.coliis a soluble 48 kDa protein that is activated in response to cellular stress, radiation and growth factors. This protein is the full-length form of the protein with a C-terminal His-tagged (His-tag on COOH terminus).
||JUN N-terminal Kinase 2 is a member of the MAP kinase family. MAP kinases act as an integration point for multiple biochemical signals, and are involved in a wide variety of cellular processes such as proliferation, differentiation, transcription regulation and development. This kinase targets specific transcription factors, and thus mediates immediate-early gene expression in response to various cell stimuli. It is most closely related to MAPK8, both of which are involved in UV radiation induced apoptosis, thought to be related to the cytochrome c-mediated cell death pathway. This gene and MAPK8 are also known as c-Jun N-terminal kinases. This kinase blocks the ubiquitination of tumor suppressor p53, and thus it increases the stability of p53 in nonstressed cells. Studies of this gene"s mouse counterpart suggest a key role in T-cell differentiation. Four alternatively spliced transcript variants encoding distinct isoforms have been reported.
|Amino Acid Sequence:
||MSDSKCDSQFYSVQVADSTFTVLKRYQQLKPIGSGAQGIVCAAFDTVLGINVAVKKLSRPFQNQTHAKRAYRELVLLKCVNHKNIISLLNVFTPQKTLEEFQDVYLVMELMDANLCQVIHMELDHERMSYLLYQMLCGIKHLHSAGIIHRDLKPSNIVVKSDCTLKILDFGLARTACTNFMMTPYVVTRYYRAPEVILGMGYKENVDIWSVGCIMGELVKGCVIFQGTDHIDQWNKVIEQLGTPSAEFMKKLQPTVRNYVENRPKYPGIKFEELFPDWIFPSESERDKIKTSQARDLLSKMLVIDPDKRISVDEALRHPYITVWYDPAEAEAPPPQIYDAQLEEREHAIEEWKELIYKEVMDWEERSKNGVVKDQPSDAAVSSNATPSQSSSINDISSMS TEQTLASDTD SSLDASTGPLEGCRGGGSHH HHHH
||Greater than 85% as determined by SDS-PAGE. Mass spectrometry of tryptic digest products for identity determination.
||Sterile Filtered clear solution.
||25mM HEPES at pH 8.0, 150mM NaCl, 2mM DTT and 10% glycerol.
||Activity is approximately 200 units/mg. A certificate of analysis is provided, stating the enzymatic activity for each production lot. 1.0 unit of activity is defined as the amount of enzyme required to catalyze the transfer of 1.0 nmole of phosphate from ATP to to a protein substrate per minute at 30ºC.
||Store at 4°C if entire vial will be used within 2-4 weeks. Store, frozen at -20°C for longer periods of time. Avoid multiple freeze-thaw cycles.