"PAK7" Related Products

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Recombinant Human PAK7 293 Cell Lysate

Cat.No.: PAK7-3453HCL
Description: Antigen standard for p21 protein (Cdc42/Rac)-activated kinase 7 (PAK7), transcript variant 1 is a lysate prepared from HEK293T cells transiently transfected with a TrueORF gene-carrying pCMV plasmid and then lysed in RIPA Buffer. Protein concentration was determined using a colorimetric assay. The antigen control carries a C-terminal Myc/DDK tag for detection.
Source: HEK 293 cells
Species: Human
Components: This product includes 3 vials: 1 vial of gene-specific cell lysate, 1 vial of control vector cell lysate, and 1 vial of loading buffer. Each lysate vial contains 0.1 mg lysate in 0.1 ml (1 mg/ml) of RIPA Buffer (50 mM Tris-HCl pH7.5, 250 mM NaCl, 5 mM EDTA, 50 mM NaF, 1% NP40). The loading buffer vial contains 0.5 ml 2X SDS Loading Buffer (125 mM Tris-Cl, pH6.8, 10% glycerol, 4% SDS, 0.002% Bromophenol blue, 5% beta-mercaptoethanol).
Size: 0.1 mg
Storage Instruction: Store at -80°C. Minimize freeze-thaw cycles. After addition of 2X SDS Loading Buffer, the lysates can be stored at -20°C. Product is guaranteed 6 months from the date of shipment.
Applications: ELISA, WB, IP. WB: Mix equal volume of lysates with 2X SDS Loading Buffer. Boil the mixture for 10 min before loading (for membrane protein lysates, incubate the mixture at room temperature for 30 min). Load 5 ug lysate per lane.
Gene Name: PAK7 p21 protein (Cdc42/Rac)-activated kinase 7 [ Homo sapiens ]
Official Symbol: PAK7
Synonyms: PAK7; p21 protein (Cdc42/Rac)-activated kinase 7; p21(CDKN1A) activated kinase 7; serine/threonine-protein kinase PAK 7; KIAA1264; PAK5; PAK-5; PAK-7; protein kinase PAK5; p21-activated kinase 5; p21-activated kinase 7; p21CDKN1A-activated kinase 7; p21(CDKN1A)-activated kinase 7; serine/threonine-protein kinase PAK7; MGC26232;
Gene ID: 57144
mRNA Refseq: NM_020341
Protein Refseq: NP_065074
MIM: 608038
UniProt ID: Q9P286
Chromosome Location: 20p12
Pathway: Activation of Rac, organism-specific biosystem; Axon guidance, organism-specific biosystem; Axon guidance, conserved biosystem; Axon guidance, organism-specific biosystem; Developmental Biology, organism-specific biosystem; ErbB signaling pathway, organism-specific biosystem; ErbB signaling pathway, conserved biosystem;
Function: ATP binding; nucleotide binding; protein serine/threonine kinase activity;

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