"PACSIN3" Related Products

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Recombinant Human PACSIN3 293 Cell Lysate

Cat.No.: PACSIN3-3472HCL
Description: Antigen standard for protein kinase C and casein kinase substrate in neurons 3 (PACSIN3) is a lysate prepared from HEK293T cells transiently transfected with a TrueORF gene-carrying pCMV plasmid and then lysed in RIPA Buffer. Protein concentration was determined using a colorimetric assay. The antigen control carries a C-terminal Myc/DDK tag for detection.
Source: HEK 293 cells
Species: Human
Components: This product includes 3 vials: 1 vial of gene-specific cell lysate, 1 vial of control vector cell lysate, and 1 vial of loading buffer. Each lysate vial contains 0.1 mg lysate in 0.1 ml (1 mg/ml) of RIPA Buffer (50 mM Tris-HCl pH7.5, 250 mM NaCl, 5 mM EDTA, 50 mM NaF, 1% NP40). The loading buffer vial contains 0.5 ml 2X SDS Loading Buffer (125 mM Tris-Cl, pH6.8, 10% glycerol, 4% SDS, 0.002% Bromophenol blue, 5% beta-mercaptoethanol).
Size: 0.1 mg
Storage Instruction: Store at -80°C. Minimize freeze-thaw cycles. After addition of 2X SDS Loading Buffer, the lysates can be stored at -20°C. Product is guaranteed 6 months from the date of shipment.
Applications: ELISA, WB, IP. WB: Mix equal volume of lysates with 2X SDS Loading Buffer. Boil the mixture for 10 min before loading (for membrane protein lysates, incubate the mixture at room temperature for 30 min). Load 5 ug lysate per lane.
Gene Name: PACSIN3 protein kinase C and casein kinase substrate in neurons 3 [ Homo sapiens ]
Official Symbol: PACSIN3
Synonyms: PACSIN3; protein kinase C and casein kinase substrate in neurons 3; protein kinase C and casein kinase substrate in neurons protein 3; SDPIII; syndapin III; SH3 domain-containing protein 6511; FLJ17861;
Gene ID: 29763
mRNA Refseq: NM_001184974
Protein Refseq: NP_001171903
MIM: 606513
UniProt ID: Q9UKS6
Chromosome Location: 11p12-p11
Function: cytoskeletal protein binding; protein binding;

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