"ODF2" Related Products

Recombinant Human ODF2 293 Cell Lysate

Cat.No.: ODF2-3598HCL
Description: Antigen standard for outer dense fiber of sperm tails 2 (ODF2), transcript variant 1 is a lysate prepared from HEK293T cells transiently transfected with a TrueORF gene-carrying pCMV plasmid and then lysed in RIPA Buffer. Protein concentration was determined using a colorimetric assay. The antigen control carries a C-terminal Myc/DDK tag for detection.
Source: HEK 293 cells
Species: Human
Components: This product includes 3 vials: 1 vial of gene-specific cell lysate, 1 vial of control vector cell lysate, and 1 vial of loading buffer. Each lysate vial contains 0.1 mg lysate in 0.1 ml (1 mg/ml) of RIPA Buffer (50 mM Tris-HCl pH7.5, 250 mM NaCl, 5 mM EDTA, 50 mM NaF, 1% NP40). The loading buffer vial contains 0.5 ml 2X SDS Loading Buffer (125 mM Tris-Cl, pH6.8, 10% glycerol, 4% SDS, 0.002% Bromophenol blue, 5% beta-mercaptoethanol).
Size: 0.1 mg
Storage Instruction: Store at -80°C. Minimize freeze-thaw cycles. After addition of 2X SDS Loading Buffer, the lysates can be stored at -20°C. Product is guaranteed 6 months from the date of shipment.
Applications: ELISA, WB, IP. WB: Mix equal volume of lysates with 2X SDS Loading Buffer. Boil the mixture for 10 min before loading (for membrane protein lysates, incubate the mixture at room temperature for 30 min). Load 5 ug lysate per lane.
Gene Name: ODF2 outer dense fiber of sperm tails 2 [ Homo sapiens ]
Official Symbol: ODF2
Synonyms: ODF2; outer dense fiber of sperm tails 2; outer dense fibre of sperm tails 2; outer dense fiber protein 2; cancer/testis antigen 134; CT134; ODF84; cenexin 1; sperm tail structural protein; outer dense fiber of sperm tails, 84-kD; ODF2/1; ODF2/2; MGC9034; FLJ44866; MGC111096;
Gene ID: 4957
mRNA Refseq: NM_001242352
Protein Refseq: NP_001229281
MIM: 602015
UniProt ID: Q5BJF6
Chromosome Location: 9q34
Pathway: Cell Cycle, organism-specific biosystem; Cell Cycle, Mitotic, organism-specific biosystem; Centrosome maturation, organism-specific biosystem; G2/M Transition, organism-specific biosystem; Loss of Nlp from mitotic centrosomes, organism-specific biosystem; Loss of proteins required for interphase microtubule organization??from the centrosome, organism-specific biosystem; Mitotic G2-G2/M phases, organism-specific biosystem;
Function: protein binding; structural molecule activity;

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