Recombinant Human NUP93 293 Cell Lysate

• Cat.No.: NUP93-3627HCL

Specification

• Description: Antigen standard for nucleoporin 93kDa (NUP93) is a lysate prepared from HEK293T cells transiently transfected with a TrueORF gene-carrying pCMV plasmid and then lysed in RIPA Buffer. Protein concentration was determined using a colorimetric assay. The antigen control carries a C-terminal Myc/DDK tag for detection.
• Source: HEK 293 cells
• Species: Human
• Components: This product includes 3 vials: 1 vial of gene-specific cell lysate, 1 vial of control vector cell lysate, and 1 vial of loading buffer. Each lysate vial contains 0.1 mg lysate in 0.1 ml (1 mg/ml) of RIPA Buffer (50 mM Tris-HCl pH7.5, 250 mM NaCl, 5 mM EDTA, 50 mM NaF, 1% NP40). The loading buffer vial contains 0.5 ml 2X SDS Loading Buffer (125 mM Tris-Cl, pH6.8, 10% glycerol, 4% SDS, 0.002% Bromophenol blue, 5% beta-mercaptoethanol).
• Size: 0.1 mg
• Storage Instruction: Store at -80°C. Minimize freeze-thaw cycles. After addition of 2X SDS Loading Buffer, the lysates can be stored at -20°C. Product is guaranteed 6 months from the date of shipment.
• Applications: ELISA, WB, IP. WB: Mix equal volume of lysates with 2X SDS Loading Buffer. Boil the mixture for 10 min before loading (for membrane protein lysates, incubate the mixture at room temperature for 30 min). Load 5 ug lysate per lane.

Gene Information

• Gene Name: NUP93 nucleoporin 93kDa [ Homo sapiens ]
• Official Symbol: NUP93
• Synonyms: NUP93; nucleoporin 93kDa; nuclear pore complex protein Nup93; KIAA0095; nucleoporin Nup93; 93 kDa nucleoporin; NIC96; MGC21106
• Gene ID: 9688
• mRNA Refseq: NM_001242795
• Protein Refseq: NP_001229724
• MIM: 614351
• UniProt ID: Q8N1F7
• Chromosome Location: 16q13
• Pathway: Antiviral mechanism by IFN-stimulated genes, organism-specific biosystem; Cytokine Signaling in Immune system, organism-specific biosystem; Disease, organism-specific biosystem; Export of Viral Ribonucleoproteins from Nucleus, organism-specific biosystem; Gene Expression, organism-specific biosystem; Glucose transport, organism-specific biosystem; HIV Infection, organism-specific biosystem

Reviews

03/03/2023

It effectively accelerates the process of chemical reaction and shortens the experimental time, which brings great convenience to our experiments.

02/23/2022

NUP93 is cost-effective and suitable for large-scale applications.

01/18/2022

The low cost of preparation, cost-effective and cost-effective make it very suitable for our research.

Q&As

How does NUP93 protein interact with other proteins?

The NUP93 protein may interact with other proteins to fulfill its function. These interactions can involve various types of protein-protein interactions like electrostatic interactions, hydrogen bonding, etc. Understanding these interactions can help unravel the mechanism of action of the NUP93 protein in cells.

What is the function of NUP93 protein?

The main function of NUP93 protein may be as a component of the nuclear pore complex, involved in the regulation of processes such as nucleoplasm exchange and signal transduction. It may perform these functions by recognizing and transporting specific substrates.

What are the patterns of NUP93 protein expression?

NUP93 protein may be highly expressed in specific tissues and cells and low in other tissues and cells. Its expression pattern may be affected by a variety of factors, including gene regulation, transcription factors, etc.

Do mutations or modifications in the NUP93 protein have an effect on the organism?

Mutations or modifications of the NUP93 protein may affect its function and thus affect the organism. The specific effects may vary depending on the type and location of the mutation or modification and may involve various biological processes, such as growth and development, immune response, etc.

In which biological processes does NUP93 protein function?

This protein may be involved in a variety of biological processes, including nucleoplasm exchange, signal transduction, cell cycle regulation, etc. These processes often require the involvement of nucleoporins to enable the transport and regulation of substances inside and outside the nucleus.

How is the expression level of NUP93 protein regulated?

The expression level of NUP93 protein may be regulated by a variety of factors, including gene transcription, translation, post-transcriptional modifications, etc. Studying these regulatory mechanisms can help to understand the expression patterns of NUP93 protein in organisms and its effects on biological processes.