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Recombinant Human NUP133 293 Cell Lysate

Cat.No.: NUP133-3633HCL
Description: Antigen standard for nucleoporin 133kDa (NUP133) is a lysate prepared from HEK293T cells transiently transfected with a TrueORF gene-carrying pCMV plasmid and then lysed in RIPA Buffer. Protein concentration was determined using a colorimetric assay. The antigen control carries a C-terminal Myc/DDK tag for detection.
Source: HEK 293 cells
Species: Human
Components: This product includes 3 vials: 1 vial of gene-specific cell lysate, 1 vial of control vector cell lysate, and 1 vial of loading buffer. Each lysate vial contains 0.1 mg lysate in 0.1 ml (1 mg/ml) of RIPA Buffer (50 mM Tris-HCl pH7.5, 250 mM NaCl, 5 mM EDTA, 50 mM NaF, 1% NP40). The loading buffer vial contains 0.5 ml 2X SDS Loading Buffer (125 mM Tris-Cl, pH6.8, 10% glycerol, 4% SDS, 0.002% Bromophenol blue, 5% beta-mercaptoethanol).
Size: 0.1 mg
Storage Instruction: Store at -80°C. Minimize freeze-thaw cycles. After addition of 2X SDS Loading Buffer, the lysates can be stored at -20°C. Product is guaranteed 6 months from the date of shipment.
Applications: ELISA, WB, IP. WB: Mix equal volume of lysates with 2X SDS Loading Buffer. Boil the mixture for 10 min before loading (for membrane protein lysates, incubate the mixture at room temperature for 30 min). Load 5 ug lysate per lane.
Gene Name: NUP133 nucleoporin 133kDa [ Homo sapiens ]
Official Symbol: NUP133
Synonyms: NUP133; nucleoporin 133kDa; nucleoporin 133kD; nuclear pore complex protein Nup133; FLJ10814; nucleoporin Nup133; 133 kDa nucleoporin; hNUP133; MGC21133;
Gene ID: 55746
mRNA Refseq: NM_018230
Protein Refseq: NP_060700
MIM: 607613
UniProt ID: Q8WUM0
Chromosome Location: 1q42.13
Pathway: Antiviral mechanism by IFN-stimulated genes, organism-specific biosystem; Cell Cycle, organism-specific biosystem; Cell Cycle, Mitotic, organism-specific biosystem; Cytokine Signaling in Immune system, organism-specific biosystem; DNA Replication, organism-specific biosystem; Disease, organism-specific biosystem; Export of Viral Ribonucleoproteins from Nucleus, organism-specific biosystem;
Function: nucleocytoplasmic transporter activity; protein binding;

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